采用常规、含Cd(2+)和含Pb(2+)的马丁氏培养基,对云南省会泽县废弃铅锌矿区和非矿区小花南芥(Arabisalpine)根际真菌进行分离,将分离的菌株接种到含不同浓度(0、0.05、0.5、5mmolL(-1))Cd(2+)的马铃薯葡萄糖培养液中,比较废弃铅锌矿区和非矿区小花南芥根际真菌的镉耐性.结果表明:Cd(2+)显著抑制铅锌矿区和非矿区小花南芥根际真菌的生长;常规、含Cd(2+)和含Pb(2+)的马丁氏培养基分离的铅锌矿区小花南芥根际真菌Cd(2+)的生长半数抑制浓度(EC50值)平均值和最大值均明显大于非矿区,表明铅锌矿区小花南芥根际真菌对Cd(2+)的耐性强于非矿区;3种培养基分离的真菌中,含Cd(2+)培养基分离的铅锌矿区小花南芥根际真菌对Cd(2+)的耐性最强.表3参26
Culturable rhizosphere fungi of Arabis alpina in abandoned lead-zinc mining and non-mining area in Huize, Yunnan, China were isolated with normal, Cd~(2+) and Pb~(2+) containing Martin’s media. Cd~(2+) tolerance of those isolated fungi were investigated with potato dextrose fluid media containing 0, 0.05, 0.5 and 5 mmol L~(-1) Cd~(2+). The results showed that the growth of rhizosphere fungi of Arenaria orbiculata in the abandoned lead-zinc mining and non-mining area was signif icantly suppressed by Cd~(2+). The mean and maximum lethal concentrations of 50% (EC50 value) of Cd~(2+) to the rhizosphere fungi in the abandoned lead-zinc mining area isolated by normal, Cd~(2+) and Pb~(2+) containing media were obviously greater than those in the non-mining area, which indicated that Cd~(2+) tolerance of the rhizosphere fungi in the abandoned lead-zinc mining area was greater than that in the non-mining area. Among these fungi isolated by these 3 media, the rhizosphere fungi of A. alpina in the abandoned lead-zinc mining area isolated by Cd~(2+) containing medium were the most tolerant to Cd~(2+). Tab3, Ref26