中文摘要：

目的：从块根芍药中分离出一株产红色素的内生菌并对其进行鉴定。方法：通过PCR方法扩增XJU-PA-6的16S rD-NA,并与GeneBank中已鉴定菌的16S rDNA序列对比,用N-J方法构建系统进化树,用Bootstraping法对其评估。同时结合其形态特征、生理生化检测、G＋C mol %含量对XJU-PA-6进行鉴定。并利用紫外-可见光谱法对XJU-PA-6产生的红色素进行分析。结果：XJU-PA-6的16S rDNA序列与EF195349 Serratia sp.同源性为99 %,G＋C mol %含量为57.8mo1 %。红色素的最大吸收波长为533.8nm。结论：将菌株XJU-PA-6鉴定为粘质沙雷氏菌。XJU-PA-6产生的红色素初步推测为灵菌红素。

英文摘要：

Objective： A red pigment- producing bacterium from Paeoruia anomala was isolated and identified.Method： 16S rDNA gene sequence was amplified by PCR. PCR products was sequenced and the DNA sequence was blasted on GeneBank. Phylogenetic tree was constructed with neighbor - joining method and the relation of XJU - PA - 6 and other Serratia sp. was evaluated by using Bootstrap, Based on the results of morphological, physiological and chemotaxonomic characteristics, and G ＋ Cmol% content, the XJU- PA- 6 was identified. Result： The sequence analysis of the 16S rDNA showed that XJU - PA - 6 was closely related to EF195349 Serratia sp., with the similarity of 99%. The G ＋ C mol% content of XJU - PA - 6 is 57.8 mol%. Condusion： The XJU - PA- 6 was identified as Serratia marcescens.