欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
桃属（Amygdalus）植物是我国重要的果树，有些种类是常见的城市绿化观赏树种，也是多种蚜虫的寄主。蚜虫的体型小，有复杂的多型现象，传统的形态学特征往往无法实现对物种的准确而快速的鉴定。本研究应用DNA条形码技术，基于cq基因序列分析，对我国桃属植物上的蚜虫进行编码，为桃属植物上蚜虫的物种快速、准确鉴定提供有力的支持。本研究共编码桃属植物上蚜虫12种，其中蚜亚科Aphidinae6属10种，毛管蚜亚科Greenideinae1属1种，毛蚜亚科Chaitophorinae1属1种。共获得cq基因序列96条，种内平均差异为0．76％，种问为5．7％～15．5％。基于CGI序列构建了NJ树，绝大多数物种的样品有效地聚为一支，且支持率达到了95％以上。结合遗传距离和系统发育树分析表明，基于cq序列的DNA条形码能有效区分99％的桃属植物上的蚜虫物种。
英文摘要：
Amygdalus plants are important fruit trees and some are common ornamental plants. They are also the host plants for a variety of aphids. It is difficult to identify aphids rapidly and accurately by traditional methods because of the degradation of specimens, specialization of morphological characters and the occurrence of multiple forms within species. In order to identify aphids rapidly and accurately, DNA barcoding based on COI sequences was used to analyze aphids on Amygdalus plants. In this study, 12 species were found, including 10 species from Aphidinae, 1 species from Greenideinae and I species from Chaitophorinae. 96 COI sequences were obtained from this study. The average intraspecific divergence was 0.76% while interspecific divergence was 5.7% -15.5%. NJ trees were built based on COI sequences, in which most samples of the same species clustered together with strong support （more than 95% bootstrap value）. Combining sequence divergences and phylogenetic tree results, 99% of the aphid species on Amygdalus plants were well-differentiated by DNA barcoding.
同期刊论文项目
同项目期刊论文
Leaf-litter thrips of the genus Adraneothrips from Asia and Australia (Thysanoptera, Phlaeothripinae
A new Shivaphis species (Hemiptera: Aphididae) on the Chinese endemic plant, Pteroceltis tatarinowii
Genus Aulacorthum Mordvilko (Hemiptera: Aphididae) from China with description of two new species an
Asiphonipponaphis, a new genus of Hormaphidinae (Hemiptera,Aphididae) causing galls on Distylium chi
Comparative analysis of mitochondrial genomes of five aphid species (Hemiptera: Aphididae) and phylo
Molecular phylogenetic evidence for paraphyly of Ceratovacuna and Pseudoregma (Hemiptera, Hormaphidi
First record of the aphid genus Jacksonia Theobald (Hemiptera, Aphididae, Aphidinae) from China, wit
First record of the genus Sinuothrips Collins in Phlaeothripinae (Thysanoptera: Phlaeothripidae) fro
Revision of Neothoracaphis Takahashi with description of a new species from China (Hemiptera: Aphidi
The genus Bactrothrips in China (Thysanoptera: Idolothripinae): morphological and molecular data, an
A new genus and species of Macrosiphini (Hemiptera, Aphididae) from China, living on Isodon eriocaly
The fern-feeder aphids (Hemiptera: Aphididae) from China: a generic account, descriptions of one new
Phylogenetic congruence between Mollitrichosiphum (Aphididae: Greenideinae) and Buchnera indicates i
New records and nomenclatural changes among spore-feeding thrips from China (Thysanoptera, Phlaeothr
Use of a mitochondrial COI sequence to identify species of the subtribe Aphidina (Hemiptera, Aphidid
Phylogeny and species differentiation of Mollitrichosiphum spp. (Aphididae, Greenideinae) based on m
Ephedraphis Hille Ris Lambers (Hemiptera: Aphididae), with a key to species and one new species from
The complete mitochondrial genome of Cervaphis quercus (Insecta: Hemiptera: Aphididae: Greenideinae)
A total-evidence phylogenetic analysis of Hormaphidinae (Hemiptera: Aphididae), with comments on the
The effectiveness of three regions in mitochondrial genome for aphid DNA barcoding: a case in Lachin
The genus Meiothrips Priesner (Thysnoptera, Phlaeothripidae, Idolothripinae) with a key and a new sp
期刊信息
位置：