中文摘要：

以食物中毒性金黄色葡萄球菌毒素为研究对象，利用PCR方法分别扩增出金黄色葡萄球菌肠毒素SEB1、SEB2、SED1、SED2、SEE基因片段。将克隆得到的5个毒素基因片段采用柔性的Linker序列（Gly4Ser）3进行串联（SEB1-SEB2-SED1-SED2-SEE），通过重叠延伸PCR法扩增出了融合毒素T，目的基因全长1835bp，测序结果同源性达99％。将融合基因克隆到pET-28a（＋）原核表达载体，转化大肠杆菌后成功表达了融合蛋白，蛋白主要以包涵体形式存在，占菌体总蛋白的29．6％。

英文摘要：

Staphylococcal enterotoxin genes SEB1,SEB2, SED1, SED2 and SEE were amplyfied by PCR and connected by a linker（Gly4 Set）3 using overlap extension PCR with the following order SEB1-SEB2-SED1-SED2-SEE, the cloned fusion gene was 1 835 bp in length,with the homologies of 99%. Then the fusion gene was cloned into expression vector pET-28a（＋）, followed by transformation of the recombinant plasmid into E. coli DH5α for expression of the fusion protein. The expressed product existed in the form of cytoryctes protein accounting for about 29.6 % of total cellular protein. The results provide a foundation for the broad spectrum detection of staphylococcal enterotoxin.