中文摘要：

利用大肠杆菌无细胞表达系统可溶性表达含his标签的水通道蛋白Z（AqpZ）,并经镍柱亲和层析纯化,浓缩得到质量浓度为490μg/mL的AqpZ。将纯AqpZ重构到DOPC脂质体中,嵌入率为57%。用停留光谱定量分析了AqpZ的滤水功能,测得其水渗透因子Pf值为（258.1±8.9）μm/s,约是对照组未嵌入AqpZ的空脂质体的2.8倍。采用静电吸附、层层自组装的方法制备嵌入AqpZ的仿生纳滤膜。用截留反渗透装置分析其水通量和NaCl截留率,结果表明：与未整合AqpZ的膜相比,嵌入含his标签AqpZ的仿生膜的水通量明显高于对照,且保持较高的截盐率。本研究为进一步研究水通道蛋白和仿生膜打下了坚实的基础。

英文摘要：

Soluble his-tag AqpZ was expressed using E. coli cell-free expression system, and was lately purified by Ni2＋ -NTA affinity chromatograph. Finally, 490 μg/mL AqpZ solution was obtained after the concentration. Then the purified AqpZ was reconstituted into DOPC liposomes with the ingerated efficiency （57%）. The water channel activity of proteoliposomes was analyzed by stopped-flow. The result shows that osmotic water permeability value of proteoliposomes was （258. 1 ± 8.9） μm/s, about 2.8 times that of the control. The his-tag AqpZ embedded layer- by-layer membrane demonstrated a higher water flux and NaCl rejection because of the presence of AqpZ. This work could be the foundation for the further study of Aquaporins and biomimetic membranes.