中文摘要：

水通道蛋白Z（AQPZ）具有水选择专一性强、渗透性高等特点,可广泛用于制备水回收和脱盐的生物仿生膜。定点突变詹氏甲烷球菌（Methanococcus jannaschii）的酪氨酸-t RNA合成酶（tyrosyl-t RNA synthetase,TyrRS）,使其可催化非天然氨基酸（丙炔氧基-L-苯丙氨酸（p-propargyloxyphenylalanine,p Ppa））合成对应氨酰t RNA。将上述转运系统与大肠杆菌无细胞体系结合,在AQPZ的特定位点引入了pPpa将改性的AQPZ（P-AQPZ）重构到1,2-二油酰基磷脂酰胆碱（1,2-dioleoyl-sn-glycero-3-phosphocholine,DOPC）,经停留光谱分析,其水渗透因子Pf值为3.42×10^-4 m·s^-1,是AQPZ的2.78倍。用截留反渗透装置分析发现P-AQPZ仿生膜的水通量高于AQPZ仿生膜并可保持较高的截盐率。

英文摘要：

Aquaporin Z （AQPZ） is a typical orthodox aquaporin with high water permeability and selectivity, and it could be widely used in biomimetic membrane for water recycling and desalination. Site-specific mutagenesis of tyrosyl-tRNAsynthetase （TyrRS） from Methanococcus jannaschii was processed to synthesize corresponding amino acyl tRNA from unnatural amino acids （p-propargyloxyphenylalanine,pPpa）. pPpa was then site-specifically incorporated into AQPZ by combining the above transfer system with a Escherichia coli cell-free system, and the modified AQPZ was reconstituted into 1,2-dioleoyl-sn-glycero-3-phosphocholine （DOPC） liposomes and analyzed by stopped-flow spectroscopy. The results show that the osmotic water permeability value （Pf） of the modified AQPZ proteoliposomes is 3.42×10^-4 m·s^-1, which is 2.78 times higher than that of the AQPZ proteoliposomes. The layer-by-layer membrane embedded with the modified AQPZ demonstrates higher water flux than the AQPZ embedded membrane with same high salt rejection rates.