本试验旨在研究过瘤胃胆碱(RPC)对成年黔北麻羊瘤胃发酵及血浆生化指标的影响。以体况良好,年龄、体重[(35.0±1.2)kg]一致的成年黔北麻羊6只为试验动物,分为对照组和试验组,每组3个重复,每个重复1只羊。采用交叉试验设计,对照组饲喂基础饲粮,不含RPC;试验组补饲10 g/d的RPC。试验持续30 d,分2期,每期15 d;每期最后1 d采集瘤胃液测定p H、氨态氮浓度、微生物蛋白产量、挥发性脂肪酸(乙酸、丙酸、丁酸)浓度、纤维素酶(微晶纤维素酶、羧甲基纤维素酶、纤维二糖酶、木聚糖酶)活性,同时采血测定血浆葡萄糖、尿素氮、总蛋白、白蛋白、总胆固醇、甘油三酯、肌酐浓度。结果显示:1)RPC对山羊采食速度没有影响,对采食量没有显著影响(P〉0.05);2)RPC对瘤胃液p H、氨态氮浓度、微生物蛋白产量、挥发性脂肪酸浓度及4种纤维素酶活性影响较小,2组间差异均不显著(P〉0.05);3)RPC对血浆生化指标影响较小,2组间差异均不显著(P〉0.05)。由此可见,补饲10 g/d的RPC对成年黔北麻羊的采食量、瘤胃发酵参数和血浆生化指标没有显著影响。
The present study was conducted to investigate the effects of rumen-protected choline( RPC) on rumen fermentation and plasma biochemical indices of Qianbeima goats. Six healthy goats were allocated to 2groups with 3 replicates per group and 1 goat per replicate using a cross-over design. Goats in control group were fed a basal diet,and those in test group were supplemented with 10 g/d RPC. The experiment lasted for30 days including 2 phases with 15-day per phase. Rumen fluid and plasma were collected at the last day of each phase. Rumen fluid p H,ammonia nitrogen concentration,microbial protein yield,volatile fatty acid( acetic acid,propionic acid and butyric acid) concentrations and cellulase( microcrystalline cellulose,carboxymethyl cellulose,cellobiase and xylanase) activities were determined; plasma concentrations of glucose,urea nitrogen,total protein,albumin,total cholesterol,triglyceride and creatinine were also determined. The results showed as follows: 1) RPC had no effect on feeding speed,and had no significant effect on feed intake( P〉0.05); 2) rumen fluid p H,ammonia nitrogen concentration,microbial protein yield,volatile fatty acid concentrations and 4 kinds of cellulase activities were not significantly affected by RPC supplementation( P 〉0.05); 3) there were no significant differences in plasma biochemical indices between control and test group( P〉0.05). It is indicated from the above-mentioned results that 10 g/d RPC supplementation has no significant effects on feed intake,rumen fermentation parameters and plasma biochemical indices of Qianbeima goats.