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体外培养单个造血干细胞实验方法的建立
  • ISSN号：1001-1978
  • 期刊名称：中国药理学通报
  • 时间：2012.3
  • 页码：435-438
  • 分类：R329.24[医药卫生—人体解剖和组织胚胎学;医药卫生—基础医学] R329.28[医药卫生—人体解剖和组织胚胎学;医药卫生—基础医学]
  • 作者机构：[1]中国医学科学院血液病医院（血液学研究所）实验血液学国家重点实验室,天津300020
  • 相关基金：国家自然科学基金资助项目（No 81170465 90913018 81090411）; 实验血液学国家重点实验室开放课题基金（ZK11-07 ZK08-04）
  • 相关项目：白血病状态下正常造血干/祖细胞的生物学行为及调控机制研究
作者： 纪庆|王金宏|许静|田晨|程辉|刘延风|张娜|程涛|高瀛岱|
关键词： 单个HSC培养, 流式细胞分选与分析, 血细胞涂片, P18, 小分子, 干细胞扩增, single hematopoietic stem cell in vitro culture, flow cytometry sorting and analysis, blood smear, p18, small molecule, HSC proliferation
中文摘要：

目的通过建立一种体外稳定培养单个造血干细胞（hematopoietic stem cell,HSC）的实验方法,在未来筛选可以促进HSC体外扩增的化合物。方法通过配制HSCs体外培养液并结合流式分选技术,我们建立了一种在体外不需要基质细胞支持的,稳定的,高通量培养单个HSC形成一个血细胞集落的实验方法。结果只需培养10～14 d即可观察到化合物对HSC的作用,并结合流式细胞分析技术替代人工计数血细胞分化情况,进一步节省人力,具有客观、重复性强的特点,满足了高通量筛选药物的要求,为后续的研究和化合物筛选工作打下基础。为了验证此方法的可靠性,使用BIO（一种通过抑制糖原合成酶激酶-3,进而可以促进HSC自我更新的化合物）时,实验结果与文献报道的研究结果一致。结论该实验方法在有关造血干细胞体外扩增的药物筛选方面具有很好的应用价值。

英文摘要：

Aim To establish a method to stably culture a single HSC in vitro, to screen the compounds which can promote the self-renew of hematopoietic stem cells （HSCs）. Methods By the configuration of HSCs in vitro culture medium and flow-cytometry sorting, a method was established to stably and highthroughput culture a single HSCs without the stroma cell support. Results The compound effect on the HSC was found inonly 10 to 14 days. The flow analysis of the differentiation, which substitutes the manual count, is a future saving of manpower. Conclusions This method is objective , reproducible and can be used in the high throughput screening of drugs.

同期刊论文项目
白血病状态下正常造血干/祖细胞的生物学行为及调控机制研究
期刊论文 217
ADAR1过表达与造血调控及白血病相关性研究
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正常造血干/祖细胞在白血病体内的受抑规律
期刊论文 139 会议论文 1
利用分子探针探索造血干细胞自我更新的信号传导机制
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期刊信息
  • 《中国药理学通报》
  • 中国科技核心期刊
  • 主管单位:中国科学技术协会
  • 主办单位:中国药理学会
  • 主编：魏伟 李俊
  • 地址：合肥市梅山路81号安徽医科大学校内
  • 邮编：230032
  • 邮箱：huanghs8@163.com
  • 电话：0551-65161222 65169603 65161221
  • 国际标准刊号：ISSN：1001-1978
  • 国内统一刊号：ISSN：34-1086/R
  • 邮发代号:26-52
  • 获奖情况:
  • 2003、2005年荣获国家期刊奖百种重点期刊奖,2006、2009、2010年被科技部中信所评为"百种中国...,2008年被中信所评为"中国精品科技期刊",2006-2008年获中国科协精品期刊工程项目资金资助,2009-2011年荣获中国科协精品期刊工程示范项目,2009、2011年获RCCSE中国"权威期刊"称号并名列药...
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  • 被引量:46533