以苦杏仁苷结构中的糖羟基作为反应位点进行生物素化修饰,经酯化、缩醛化、Sonogashira偶联等反应合成了一个新型的生物素标记的苦杏仁苷活性探针,其结构经1H NMR,13C NMR,IR和HR-ESI-MS表征。
The biotin-labeled amygdalin probe was successfully synthesized by esterification,acetalization and Sonagarshia coupling,using the hydroxyl group as reactive sites to modify with biotin.The structures were characterized by1 H NMR,13C NMR,IR and HR-ESI-MS.