中文摘要：

天然孕甾烷-17（20）-烯中C-17位双键的构型对分子的药理性质有重要的影响,但是采用化学位移比较等方法判断其构型存在费时以及区分模糊等缺点.该文以E-和Z-gugguls-terone为模型化合物,利用一维选择性NOE实验来进行天然孕甾烷-17（20）-烯中C-17位双键的构型测定.由于空间相对位置受到构型影响,选择反转20位的质子时,如果选择性NOE谱中仅能检测到21位甲基氢,表明该化合物为E-构型;如果除了21位甲基氢外,18位甲基氢和12位氢也存在于选择性NOE谱中,则表明该化合物为Z-构型.实验结果表明该方法具有区分度大,简单快速等优点,可以普遍适用于这些孕甾烷类化合物17位双键构型的确定.

英文摘要：

The configuration of carbon-17 ethylene bond in pregn-17（20）-ene derivatives has important influence on the pharmacological properties of the compounds.However,the previously used methods for configuration determination of pregn-17（20）-ene derivatives either are time consuming or cannot obtain certain results.In this paper,we proposed to use 1D selective NOESY to determine the configuration of carbon-17 ethylene bond in E-and Z-guggulsterone.The results showed that,by selective inversion of H-20,the NOE spectra of E-guggulsterone showed only the signal from H-21,while that of Z-guggulsterone showed additional signals from H-18 and H-12.Our results indicated that the selective NOE experiment is a rapid and simple method for configuration determination of the carbon-17 ethylene bond in pregnanes.