中文摘要：

采用了650nm红色发光二极管（LED）的矩阵（6×8）作为激发光源,构建了一种基于MINI-PAM荧光仪的调制脉冲式在线检测叶绿素荧光系统。利用该系统分别对番茄苗期、花期、果期活体叶片的荧光强度与激发光源（LED）光强关系进行试验研究。通过对可编程电源来调节LED光强（在0～1 700μmol/m2.s之间）,以50mA为步进值依次增加,并利用Excel软件对测得的荧光值F进行分析。结果显示：电流值在3 700mA以下,每一时期植株叶片的荧光强度与恒流源输出电流强度具有显著的线性关系,决定系数R2〉0.97。以此研究为基础,建立了荧光强度与激发光强度的关系模型,模型可靠性高,能够比较真切地反映出荧光强度与激发光强之间的关系。

英文摘要：

In this paper,with the 650 nm red LED matrix（6×8）was chosen as excitation source,a new measurement and control system for chlorophyll fluorescence is proposed,which is based on MINI-PAM.The relationship between chlorophyll fluorescence intensity of tomato leaf during seedling,flower and fruit in vivo and stimulate light intensity was studied.LED light intensity has been increased between 0-1700μmol/（m2·s） for the step by 50mA,regulating the programmable power.The measured fluorescence value F was calculated and analyzed by Excel.The results showed that there was a very significant linear correlation between the fluorescence intensity of each period leaves and current strength under 3700mA.The coefficient of determination： R20.97.Based on those results,a mathematical model with the change of fluorescence intensity along with excitation power was established.The established model was reliable,and it exactly reflected the relationship between fluorescence intensity and excitation power.