中文摘要：

目的：研究特异AT序列结合蛋白2（Special AT—richsequence—binding protein2，SATB2）在骨形态发生蛋白9（bone morphogenetic proteins9，BMP9）诱导的间充质干细胞（mesenchymal stem cells，MSCs）C2C12成骨分化中的作用。方法：首先用Ad—BMP9感染C2C12细胞，RT-PCR检测SATB2在基因水平上的表达变化，Westernblot检测SATB2在蛋白水平上的变化；构建过表达SATB2重组腺病毒Ad—SATB2，感染C2C12细胞后，Westernblot验证Ad—SATB2表达情况；用Ad—SATB2处理BMP9诱导的C2C12细胞，碱性磷酸酶（ALP）活性和染色检测成骨早期ALP的变化，茜素红S染色检测成骨晚期指标钙盐沉积的变化。结果：Ad—BMP9处理C2C12细胞后，比对照组SATB2在基因水平和蛋白水平上表达量上调；Ad—SATB2可以在细胞中成功表达SATB2蛋白；用Ad．SATB2处理BMP9诱导的C2C12细胞后，ALP以及钙盐的沉积与对照比较均上调。结论：SATB2可以促进BMP9诱导的间充质干细胞C2C12的成骨分化。

英文摘要：

Objective ： To analyze the effect of SATB2 on BMP9-induced osteogenic differentiation of C2C12 mesenchymal stem cells. Methods： C2C12 cells were treated with ad-BMP9, and then the expressions of SATB2 at the gene and protein level were detected with RT-PCR and Western blot, respectively. Afterwards C2C12 cells were treated with ad-SATB2 or/and BMP9, then ALP activity was detected by quantitative and staining assay, and calcium deposition was detected by Alizarin Red S staining. Results： BMP9 can enhance the expression of SATB2 at the gene level and at the protein level in C2C12 cells. Both ALP activity and calcium deposition of C2C12 cells treated with adenovirus SATB2 or/and BMP9 expressed higher than those of controls. Conclusions： SATB2 can promote BMP9-induced osteogenic differentiation of C2C12 mesenehymal stem cells