中文摘要：

目的：研究血红素加氧酶1（hemeoxygenase 1,Hmox1）在骨形态发生蛋白9（bone morphogenetic proteins 9,BMP9）诱导下间充质干细胞C3H10T1/2向成骨分化过程中发挥的作用。方法：用Ad-BMP9感染C3H10T1/2,分别用Q-PCR和Western blot检测Hmox1mRNA和蛋白水平的变化;Hmox1激动剂COPP处理BMP9诱导的C3H10T1/2细胞,碱性磷酸酶（ALP）染色和活性测定检测早期成骨指标ALP的变化;过表达Hmox1的重组腺病毒（Ad-Hmox1）处理BMP9诱导的C3H10T1/2细胞,ALP染色和活性测定早期成骨指标ALP,茜素红染色检测晚期成骨指标钙盐沉积,Western blot检测成骨相关基因COL1A1。结果：Ad-BMP9感染C3H10T1/2后,Hmox1的mRNA及蛋白水平均升高;BMP9与Hmox1激动剂COPP联用与BMP9组相比ALP的活性增强;Ad-Hmox1可以增强BMP9诱导下C3H10T1/2细胞的ALP活性和钙盐沉积,以及成骨相关基因COL1A1表达。结论：Hmox1可以促进BMP9诱导下C3H10T1/2细胞的成骨分化。

英文摘要：

Objective： To study the effect of Hmoxl on BMP9-induced osteogenesis in C3H10T1/2. Methods： First of all, C3H10T1/2 were treated with Ad-BMP9, then the transcription and translation level of Hmoxl in C3H10T1/2 were detected with Q-PCR and Western blot. Subsequently, C3H10T1/2 were treated with Hmoxl agonist COPP and BMP9 or treated with Ad-Hmoxl or/and BMP9, ALP activity was detected by quantitative and staining assay at 5 day, Calcium deposition was assayed by Alizarin Red Staining at 14 day. At last, expression of COL1A1 was measured by Western blot. Results： C3H10T1/2 infected by Ad-BMP9 results in elevated gene and protein level of Hmoxl. Ad-Hmoxl and Hmoxl agonist could enhance BMP9-induced ALP activity in C3H10T1/2. The expression of COL1A1 and calcium deposition were increased by Hmoxl. Conclusion： Hmoxl can promote BMPg-induced osteogenic differentiation in C3H10T1/2.