实验以不同生长发育阶段的款冬花序芽突起为材料,通过制作石蜡切片,在显微镜下观察款冬花序芽分化各阶段的形态特征。结果表明:款冬花序芽从7月上旬开始花序(盘)分化至十月初小花胚珠分化完成,分化时期可分为分化前期、花盘形成期、花原基分化期、中央花(筒状)花瓣原基分化期、中央花雄蕊原基分化期、中央花雌蕊原基分化期、边缘花(舌状)花瓣原基分化期、边缘花雌蕊原基分化期、中央花花粉分化形成期、子房胚珠分化期共10个时期,阐明了款冬花序芽分化各时期与生长时间的关系。
Bud primordia of Tussilago farfara L.inflorescence at various growth stages were used as experiment materials in this study.By means of paraffin sections,the morphological process of bud differentiation was observed by optical microscope.The results showed that inflorescence differentiation started in the early July and completed in the early October.The whole differentiation process could be divided into ten stages(phases): pre-differentiation,disc formation,inflorescence primordium differentiation,petal primordium differentiation of central flowers(tubiform florets),stamen primordium differentiation of central flowers,pistil primordium differentiation of central flowers,petal primordium differentiation of marginal flowers(ligulate flowers),pistil primordium differentiation of marginal flowers,pollen differentiation of central flowers,ovary and ovule differentiation.The relationship between bud differentiation and the growth period of T.farfara L.was also discussed.