中文摘要：

为了研究不同来源款冬花药材之间的质量差异，本研究采用NMR代谢组学技术同时结合HPLC含量测定，对21份不同来源款冬花药材进行化学比较。主成分分析结果显示，款冬花栽培品和野生品可明显区分，说明两者化学组成存在一定差异。然后通过有监督的偏最小二乘法判别分析（PLS-DA）确定两者的成分差异。结果显示，野生品中咖啡酸、绿原酸、芦丁、款冬酮、甲基丁酸-3，14．Z-去氢款冬素酯（EMDNT）、款冬巴耳二醇（bauer．7．ene-3β-16a-diol）、谷甾酮等次级代谢产物含量高于栽培品，这与传统经验认为的野生品质量较优一致。肝毒性吡咯里西啶生物碱肾形千里光碱（senkirkinel在不同来源款冬花药材中也存在较大差异，相关性分析结果显示，该毒性成分与3，5-二咖啡酰基奎尼酸、3，4-二咖啡酰基奎尼酸、4，5-二咖啡酰基奎尼酸、芦丁、山柰酚类似物等次级代谢物存在正相关，且其相关性具有统计学意义（P〈0．05）。款冬花药材中毒性成分和活性成分的相关性值得进一步深入研究。

英文摘要：

1H NMR-based metabolomic approach combined with multivariate statistical analysis was used to evaluate the quality of 21 Farfarae Flos （FF） samples from different growth regions. Principal component analysis showed that wild and cultivated FF could be separated clearly, suggesting a big chemical difference existed between them. Supervised PLS-DA analysis indicated that the wild samples showed higher levels of secondary metabolites, such as bauer-7-ene-3β, 16a-diol, chlorogenic acid, rutin, 7-（3＇-ethylcrotonoyloxy）-I a- （2＇-methyl-butyryloxy）-3, 14-dehydro-Z-notonipetranone （EMDNT）, tussilagone, fl-sitosterol and sitosterone. This is consistent with traditional experience that the quality of wild samples are better than that of cultivated ones. The content ofpyrrolizidine alkaloids senkirkine also differed greatly among samples from different habitats. The Pearson correlation analysis showed that senkirkine is positively correlated with 4, 5-O-dicaffeoylquinic acid, 3, 5-O-dicaffeoylquinic acid, 3,4-O-dicaffeoylquinic acid, rutin, kampferol analogues, to a statistically significant extent. The correlation between the toxic compounds and the bioactive components in FF should be further studied.