中文摘要：

【目的】探讨香樟涌金茎段组培快速繁殖方法,为工厂化生产香樟涌金组培苗提供技术支持。【方法】以香樟涌金带腋芽茎段（长1.0~1.5 cm）为外植体,进行腋芽诱导、丛生芽增殖及植株再生研究。【结果】香樟涌金腋芽在诱导培养基MS＋6-BA 1.0 mg/L＋IBA 0.02 mg/L中萌发率达100.0%,并能正常伸长展叶;在MS＋6-BA 3.0 mg/L＋IAA 0.05mg/L培养基中腋芽能正常生长并增殖,平均有效芽数6.5个;在1/2MS＋IBA 0.05 mg/L＋NAA 0.03 mg/L生根培养基中培养30 d后,有87.6%的丛生芽生根,移栽成活率达90.0%。【结论】MS＋6-BA 1.0 mg/L＋IBA 0.02 mg/L、MS＋6-BA 3.0mg/L＋IAA 0.05 mg/L、1/2MS＋IBA 0.05 mg/L＋NAA 0.03 mg/L可分别作为香樟涌金茎段腋芽最佳的诱导培养基、适宜的继代与增殖培养基和相对适合的生根培养基。

英文摘要：

[Objective ]A micropropagation protocol was developed for Cinnamomum camphora Yongjin to provide technical support for large-scale multiplication of C. camphora Yongjin by tissue culture. [Method]Using auxiliary stems （1.0-1.5 cm） of Cinnamomum camphora Yongjin as explants ,adventitious bud induction, proliferation, rooting and so on were the focal points in the current research. [Result]Bud inducement was initiated on the MS medium containing 1.0 mg/L 6-BA and 0.02 mg/L IBA. The inducement rate of valid buds reached 100%. The shoots were sub- cultured and multiplied on the improved MS medium containing 3.0 mg/L 6-BA and 0.05 mg/L IAA and the mean effective bud per shoot was 6.5. Harvested shoots were rooted in vitro in 1/2MS supplemented with 0.05 mg/L IBA and 0.03 mg/L NAA for 30 days and the rooting rate was 87.6% .The survival rate was 90.0% when the rooting shoots were moved to the greenhouse. [ Conclusion ]The combination of 6-BA （1.0 mg/L） and IBA（O.02 mg/L）is suitable to bud in- duction and growth for Cinnamomum camphora Yongjin, and the combination of 6-BA（3.0 mg/L）and IAA（0.05 mg/L） is beneficial to bud propagation. The combination of NAA（0.03 rag/L） and IBA （0.05 mg/L） is advantageous to rooting.