中文摘要：

目的：探讨核因子KB（NF-KB）在蛋白激酶C（PKC）致哮喘模型大鼠气道平滑肌细胞（ASMCs）增殖中的作用。方法：16只Wistar大鼠随机分为哮喘组（8只）和对照组（8只）。应用PKC激动剂PMA和NF-KB抑制剂PDTC干预哮喘组和对照组大鼠ASMCs。采用流式细胞术、MTT法及增殖细胞核抗原（PCNA）免疫荧光技术等方法检测ASMCs增殖；免疫荧光技术及电泳迁移率改变分析（EMSA）检测NF-KB活性。结果：PMA干预哮喘组ASMCs后S期细胞比例、A值、PCNA表达率、NF-KB；065阳性率及结合带的灰度值均显著高于未干预的哮喘组ASMCs（均P〈0．05），PDTC预处理后再给予PMA上述指标均低于仅用PMA干预及未干预者（均P〈0．05）。仅用PDTC处理的哮喘组ASMCs上述指标均低于未干预的ASMCs（均P〈0．05）。结论：NF-KB参与哮喘大鼠ASMCs增殖，在其增殖中存在着PKC-NF-KB信号途径。

英文摘要：

AIM ： To investigate the effect of protein kinase C （PKC） - nuclear factor - KB （ NF - KB） signal pathway on proliferation of airway smooth muscle cells （ASMCs） in asthmatic rats. METHODS： （1） 16 Wistar rats were divided into asthmatic group （8 rats） and control group （ 8 rats）. ASMCs from asthmatic group and control group were treated with PKC agonist PMA and NF - KB inhibitor PDTC. The proliferation of ASMCs was examined by cell cycle analysis, MTT calorimetric assay and proliferating cell nuclear antigen （PCNA） immunofluorescence staining, respectively. NF - KB activity was detected by NF - KB p65 immunofluorescence staining and electrophoretic mobility shift assay （ EM-SA）, respectively. RESULTS： The percentage of S phase, A value, the positive expression rate of PCNA, the positive expression rate of NF - KB p65 and EMSA value in asthmatic ASMCs treated with PMA were higher than those in asthmatic ASMCs without treatment （P 〈 0. 05）. After asthmatic ASMCs previously treated with PDTC, then with PMA, the above figures were lower than those in asthmatic ASMCs only treated with PMA and without treatment （P 〈 0.05）. The above figures in asthmatic ASMCs only treated with PDTC were lower than those in asthmatic ASMCs without treatment （ P 〈 0. 05）. CONCLUSION： NF - KB may contribute to the proliferation of ASMCs in asthmatic rat, in which PKC - NF - KB signal pathway is involved.