中文摘要：

目的探讨纤维连接蛋白（FN）、Ⅰ型胶原蛋白（ColⅠ）两种细胞外基质（ECM）成分对被动致敏的人气道平滑肌细胞（HASMCs）免疫功能的影响及磷脂酰肌醇-3激酶（P13K）在此调控中的作用。方法将HASMCs接种于FN、ColⅠ包被的培养板和空白培养板中，用10％哮喘患者血清被动致敏HASMCs，以10％非哮喘者血清为对照，在加入血清前用PBK抑制剂（LY294002）预处理HASMCs 30min。采用RT—PCR法检测HASMCs RANTES（regulated upon activation，normal T cell expressed and secreted）、Eotaxin、TGF—β1 mRNA表达；ELISA法测定HASMCs培养上清中RANTES、Eotaxin、TGF—β1蛋白水平。结果与单纯对照血清组比较，单纯哮喘血清组、对照血清＋FN组、对照血清＋ColⅠ组HASMCs RANTES、Eotaxin、TGF-β1 mRNA和HASMCs培养上清中蛋白的表达均增高（P〈0．05）。哮喘血清＋FN、哮喘血清＋ColⅠ处理HASMC后，RANTES、Eotaxin、TGF—β1 mRNA和HASMCs培养上清中蛋白的表达均高于单纯哮喘血清组，且LY294002干预后上述各指标均下降（P〈0．05）。结论细胞外基质对被动致敏的HASMCs免疫功能具有调控作用，PI3K可能参与细胞外基质对被动致敏的HASMCs的免疫功能调控。

英文摘要：

Objective To investigate the regulatory effects of extracellular matrixes, including fibronectin（FN） , and collagen Ⅰ （ Col Ⅰ ） on the immunologic function of human airway smooth muscle cells （HASMCs） passively sensitized with asthmatic serum, and the role of phosphoinositide 3-kinase （ PI3 K）. Methods Primarily cultured HASMCs were inoculated on the blank plates or on the plates coated with difference matrix proteins, added 10% asthmatic serum to passively sensitized non-asthmatic HASMCs and 10% non-asthmatic serum treated HASMCs as control, cell pretreated with PI3K inhibitor LY294002 for 30 min. The expressions of RANTES, Eotaxin, TGF-β1 mRNA were observed by RT-PCR and RANTES, Eotaxin, TGF-β1 protein in the cell culture supernatants was detected by enzyme-linked immunosorbent assay （ELISA）. Results Compared with the control serum group, the expressions of RANTES, Eotaxin, TGF- β1 mRNA of HASMCs and those protein in HASMCs culture supernatants were significantly increased in the asthmatic serum group and the control serum ＋ FN group and the control serum ＋ Col Ⅰ group （ P 〈 0.05 ）. The expressions of RANTES, Eotaxin, TGF-β1 mRNA of HASMCs and protein in HASMCs culture supernatants were significantly increased in the asthmatic serum ＋ FN group and the asthmatic serum ＋ Col Ⅰ group. 50 μmol/L LY294002 could significantly inhibit the expressions of RANTES, Eotaxin, TGF-β1 mRNA of HASMCs and protein in HASMCs culture supernatants（P 〈 0.05 ）. Conclusion These results suggest extracellular matrixe may regulate immunomodulatory function of HASMCs passively sensitized with asthmatic serum and PI3K signaling pathway may play an important role in the process.