中文摘要：

为建立传染性法氏囊病的动物模型,将法氏囊病病毒（IBDV）BC6/85毒株人工感染5周龄的SPF鸡,采用组织病理学和免疫组织化学的方法,用打分的形式研究攻毒后2～432h,感染鸡法氏囊中病毒的定位以及法氏囊组织病变和IBDV免疫反应阳性细胞的变化过程。结果显示感染鸡的法氏囊与法氏囊腔中都能检测出病毒和有病理损伤的现象,且IBDV检出的时间迟于损伤出现的时间。法氏囊小结中IBDV免疫反应阳性细胞密度和损伤程度的变化趋势有一定的规律性,表现出先协同增长后有所不同的变化趋势。并在感染后期发现感染鸡法氏囊中仍能检测出病毒且有少量新生的淋巴小结。通过详细记录鸡感染IBDV BC6/85后的整个病程,初步建立了该毒株的动物模型,并通过打分的形式,将感染鸡法氏囊中的病毒增殖与炎症损伤两者联系起来分析,进一步探讨了该病的致病机制并为其今后的研究提供基础数据和理论依据。

英文摘要：

To establish the animal model of infectious bursal disease and provide the data base and basic theories of this diseases,5 weeks old SPF chickens were infected by a IBDV strain BC6/85,histolpathological and Immunohistochemistrical methods were used to study the change of the pathological lesions and IBDV immunoreactive positive cells in bursa of fabricius from 2 to 432 h after infection.Results showed that IBDV and pathological lesion could be observed in the chamber and follicles of the bursa of fabricius in infected chickens and the IBDV detected after lesions appearing.Both of the pathological lesion and IBDV immunoreactive positive cells in bursa of fabricius increased at the beginning and then became different because of the positive cells disapeared with the disapearances of the bursal follicles.IBDV and bursal follicles still can be detected in the busal of infected chickens in the later period of infection.The study detail recorded the whole phases of the disease of the chickens infacted IBDV strain BC6/85 and installed the preliminary animal model.Then the form of marking was used to analyze the histological bursa lesions together with the density of IBDV immunoreactive positive cells,and searched mechanism of pathogenic