为了探讨奇球菌对铀的吸附特性,研究了pH值、吸附时间、菌体浓度、铀起始浓度和预处理奇球菌对铀吸附的影响,并考察了菌体上铀的解吸作用。结果表明,在pH值为4.0时,吸附量最大;30 min吸附基本达到平衡;吸附量与菌体浓度负相关,与铀起始浓度正相关,吸附量最大可达126.27 mg/g。经乙醇预处理的菌体,其吸附量有所上升。解吸试验表明,0.5 mol/LNa2CO3对铀的解吸率可达93.15%。与Langmuir吸附模型相比,奇球菌对U(Ⅵ)的吸附更符合Freundlich吸附等温式。
The present paper is to introduce our study of the biosorption of uranium by Deinococcus radiodurans, the effect of pH, contact time, the initial U ( Ⅵ ) concentration, the concentration of D. radiodurans, as well as the various pretreatment procedures on the biosorption in addition to the desorption efficiency were studied. The results of our research have shown that the maximal adsorption quantity can reach 48.62 mg/g with the optimal pH value being 4.0.The absorption rate can be expected to reach 95.12% when the absorption gets balanced in about 30 min. And, then, it would turn to be a dynamic adsorption-desorption process. The adsorption quantity proves in negative correlation with the concentration of D. radiodurans when the adsorption rate reaches 95.13% at 5 mg/L of the cell biomass of D. radiodarans. On the other hand, the adsorption quantity can also be expected to be positively correlated with the concentration of U( Ⅵ ), with its highest degree being over 126.27 mg/g. The results of different pretreatments indicate that the adsorption degree with ethanol pretreated biomass can be higher beyond the control level, if the adsorption quantity can increase from 46.66 mg/g to 47.74 mg/g. Meanwhile, the desorption of U(Ⅵ) from D. radiodurans cells can be very high in Na2CO3 and NaHCO3 solution, the desorption rate of 0.5 mol/L Na2CO3 solution reached 93.15% . In addition, the adsorption isotherm of U(Ⅵ) on D. radiodurans can be in good agreement with Freundlich equation (correlation coefficient = 0.999 4), that is, better than with Langmuir equation. Therefore, the absorption process is by nature not merely a single molecular change on the surface adsorption.