中文摘要：

采用反相高效液相色谱一紫外检测法，测定了罗非鱼血浆、皮肤、肌肉、心肌和大脑中邻苯二甲酸二乙酯（DEP）含量，并研究了DEP在罗非鱼体内代谢动力学和残留特征。将DEP以20mg／kg灌服罗非鱼后，取样测定不同时间点血浆和组织中的DEP浓度，并利用3p97药物动力学软件并结合Excel2003软件对实验数据进行处理和分析。结果表明，罗非鱼灌服DEP后体内吸收较快，吸收半衰期为0．12h，分布半衰期为0．27h，消除半衰期为28．09h，血浆浓度．时间曲线下面积（AUC）为52．05μg·h／ml，达峰时间为0．28h，达峰浓度为7．47μg／ml；同时在1、2、8、24、48、72、120、360h各点皮肤、肌肉、心肌和大脑中DEP浓度均比血浆中高，DEP在肌肉、皮肤、脑和心肌中的消除半衰期分别为111．77h、108．28h、99．00h和73．72h。代谢动力学和残留研究显示，DEP在罗非鱼体内吸收和分布较快，消除相对较缓慢。

英文摘要：

DEP concentrations were determined kg by reversed-phase high performance liquid chromatography （RP-HPLC） after a single oral administration at 20mg/kg in Tilapia （Oreochromis niloticus×O, aureus） plasma and edible tissues, including skin, muscle, brain and heart. After a simple liquid-liquid extraction and solid phase extraction （SPE） treatment, DEP were separated on a SHIMADZU ODS C18 column （4.6mm× 150mm, 5μm）, with acetonitrile-water （40 ： 60） elution at a flow rate of 1.0ml/min and UV at 235nm. Calculations of metabolic kinetics and residue concentrations of DEP were performed with Software 3p97 and Excel 2003. Linear calibration curves were obtained in the concentration range of 0.05--10.0μg/g （r〉0.99） and the limit of detection was 0.05μg/ml or μg/g. The recoveries were more than 85.0% and the intra- and inter-day coefficients of variation were less than 15.0% in all cases. The concentrations of DEP could be still detected at 15d after oral administration. And the results of metabolic kinetics and residues showed that DEP was absorbed quickly and eliminated slowly in Tilapia. The TI/2Ka, T1/2α and T1/2β were 0.12, 0.27, and 28.09h, respectively. The AUC, Tpe,k and Cmax were 52.051μg· h/ml, 0.28h and 7.47μg/ml. At 1, 2, 8, 24, 48, 72, 120, and 360h, the concentrations of DEP were higher in the edible tissues than those in plasma. The elimination half-life of DEP was 111.77, 108.28, 99.00 and 73.72h in skin, muscle, heart and brain, respectively. In conclusion, with our specific, simple, sensitive and accurate RP-HPLC method for determining of DEP in the plasma and edible tissues of tilapia, we found that DEP absorption and distribution were more quickly than elimination, and therefore, DEP can be accumulated in certain organs or tissues of Tilapia.