中文摘要：

采用反相高效液相色谱．紫外检测法测定了黄颡鱼血浆、肝脏中儿茶素EGCG含量，并研究了不同水温（10℃和20℃）下EGCG在黄颡鱼体内代谢动力学和肝组织消除特征。结果表明，高温下黄颡鱼腹腔注射EGCG后体内吸收较快，T1/2ka为0．21h，T1/2a小为2．07h，T1/2β为22．89h，AUC为5195．41gg·h／ml，Tp为0．95h，Cmax为257．75gg／ml；低温下分别为5．67h、9．53h、207．49h、17283．031．μg·h／ml、12．56h和117．16p．g／ml。同时在0．5、1、2、4、8、12、24、48、96h各时间点检测了肝脏组织中EGCG浓度，并计算得出高温下EGCG在肝组织中的T1／2β为24．15h，低温下肝组织中Tl／2B为64．17h。对不同水温下代谢动力学和肝组织消除规律的研究表明，EGCG在黄颡鱼体内吸收、分布和消除速度与水温关系密切，高温下均较低温下迅速。

英文摘要：

The epigallocatechin gallate （EGCG） concentrations in plasma and liver tissues were determined by the re- versed-phase high performance liquid chromatography （RP-HPLC） after a single intraperitoneal injection at 150mg/kg for Darkbarbel catfish, Pelteobagrus vachelli. The results showed that EGCG was absorbed and eliminated very quickly in high temperature （20℃）. However, EGCG was absorbed and eliminated very slowly in low temperature （10℃）. The T1/2Ka, T1/2a and T1/2β were 0.21h, 2.07h and 22.89h at 20℃, respectively. And the corresponding values were 5.67h, 9.53h and 207.49h at 10℃, respectively. The AUC, Tp and Cmax were 5195.41gg.h/ml, 0.95h and 257.75gg/ml at 20℃. Then all the values were 17283.031ag.h/ml, 12.56h and 117.16gg/ml at 10℃, respectively. The elimination half-life of EGCG in liver was 24.15h at 20℃, while the value was 64.17h at 10℃. Therefore, these results indicate that EGCG absorption, distribu- tion and excretion at 20℃ were more quickly than at 10℃. And the differences of metabolic kinetics and liver elimination of EGCG were significant at 20℃ and at 10℃.