为筛选引诱天敌昆虫的化学信息素,以应用其防控茶刺蛾(Iragoides fasciata Moore),研究了茶树[Camellia sinensis (L.) O. Kuntze]被茶刺蛾危害后释放出的挥发性有机物的变化. 应用同时萃取蒸馏法收集被茶刺蛾危害和未危害的茶叶的挥发性有机化合物,气相色谱/质谱联用仪(GC/MS)分析结果发现,未受害枝叶挥发性有机化合物共有63种组分,主要由酯类、醇类、烃类、酮类、杂环化合物、醛类、有机酸和醚类组成,烃类物质的相对含量占19.05%,而受害枝叶释放出的挥发物中烃类物质的相对含量增加到26.79%,反式-橙花叔醇、雪松醇、邻苯二甲酸二丁酯、邻苯甲酸二异丁酯相对含量显著增加,此外,产生了32种新的组分,包括10种酯类、5种醇类、5种烯烃类、4种杂环类、3种芳香烃类、3种烷烃类、1种醛和1种酮. 图2 表1 参32
The effect of the damaging of Iragoides fasciata Moore on the volatile organic compounds (VOCs) of Camellia sinensis (L.) O. Kuntze was studied. VOCs from the undamaged and damaged tea plants were collected by simultaneous distillation extraction and analyzed by gas chromatography/mass spectrometry (GC-MS). The results showed that a total of sixty-three components, mainly esters, alcohols, hydrocarbons, ketones, heterocyclic compounds, aldehydes, organic acids and ethers were identified in the undamaged twigs and leaves, and the relative amount of hydrocarbons was 19.05%, while that from the damaged ones increased to 26.79%, and the relative amount of cis-nerolidol, cedar alcohol, dibutyl phthalate and phthalic acid was significantly increased. Furthermore, thirty-two new compounds were identified in the damaged ones, including ten esters, five alcohols, five olefins, four heterocyclics, three aromatic hydrocarbons, one alkane, one ketone and one aldehyde. The results can lay a foundation for screening infochemicals which can be used as a lure for natural enemies of insects and applied to control I. fasciata Moore. Fig 2, Tab 1, Ref 32