中文摘要：

目的通过邻苯二甲酸二（2-乙基）己酯（DEHP）影响小鼠淋巴瘤细胞（EL4）分泌白细胞介素-2（IL-2）蛋白影响机制研究,探讨DEHP对辅助性T细胞1（Th1）型细胞因子影响。方法不同浓度DEHP染毒以25 ng/mL佛波酯（PMA）＋1μg/mL离子霉素（Ion）激活的EL4细胞,并用钙调神经磷酸酶抑制剂FK506进行干预 实验6 h用逆转录聚合酶链反应（RT-PCR）检测活化T细胞核因子（NFAT）的mRNA表达 实验48 h用酶联免疫吸附试验（ELISA）检测细胞上清液中IL-2蛋白表达。结果与激活剂组的（72.59±9.05）pg/mL比较,10,50μmol/L DEHP染毒组的IL-2蛋白分泌量为（36.00±5.10）,（32.00±1.86）pg/mL,明显降低EL4细胞分泌IL-2蛋白（P〈0.01） 50μmol/L DEHP明显升高了EL4细胞中NFAT2 mRNA相对含量,且降低了NFAT1 mRNA相对含量（P〈0.05） 0.05,0.5μmol/L FK506未明显影响IL-2蛋白表达 Spearman相关分析表明,DEHP影响IL-2蛋白与NFAT mRNA表达无相关。结论DEHP可能是通过NFAT非依赖途径影响EL4细胞分泌IL-2蛋白。

英文摘要：

Objective To determine the effect of di-2-ethylhexyl phthalate （DEHP） on interleukin-2（IL-2）in mouse T-cell lymphoma （ EL4 ） cells in order to study the effect of DEHP on Thl-type cytokines. Methods Propylene glycol methyl ether（25ng/mL） and Ion（ 1 μg/mL） were used to activate EL4 cells, which were exposed to the different concentrations of DEHP（ 1,10,50 μmol/L） with or without calcium-regulated neurol phosphatase inhibitor（ FK506）. Nuclear factor of activated cells（NFAT） mRNA was measured with reverse transcripdon-polymerase chain reaction （RT-PCR） at 6 hr and IL-2 protein was determined in cell supernatant with enzyme-linked immunosorbent assay （ELISA） at 48 hr. Results The DEHP of 10 and 50 μmol/L significantly reduced IL-2 protein in supernatant （ P 〈 0. 01 ）. The DEHP of 50 μmol/L increased NFAT2 mRNA expression and decreased NFAT1 mRNA expression （ both P 〈 0. 05 ）. FK506 at concentration of 0.05 and 0. 5μmol/L didn＇t express obvious effect on IL-2 protein（P 〉0.05）. There was no significant correlation between IL-2 protein and NFAT mRNA expression through Spearman correlation analysis. Conclusion DEHP affects IL-2 protein through non-dependent NFAT pathway in EL4 cells.