中文摘要：

microRNAs 是搁浅单人赛的，调整基因表示的非编码的 RNA。microRNA-16 家庭被报导了涉及房间周期规定，它能另外多重支持增长的基因的 downregulate 表示。现在的结果在当房间在甲基 methanesulfate (公里) 下面在 S 阶段期间被逮捕时，房间增加了的 HeLa 表明了那 miR-16 表情处理。这进一步导致了目标蛋白质 CDC25A 的 downregulation，而当 HeLa 房间在对待公里的 G0/G1 或 G2/M 阶段期间被逮捕时， miR-16 表示没增加。而且，当 HeLa 房间与 hydroxyurea 处理在 S 阶段期间被逮捕时， miR-16 表示没增加。这些结果建议在哺乳动物的房间的 microRNAs 的表达式层次精巧地在可变细胞的条件下面被调整。

英文摘要：

microRNAs are single-stranded, non-coding RNAs that regulate gene expression. The microRNA-16 family has been reported to be involved in cell-cycle regulation, which could also downregulate expression of multiple pro-proliferation genes. The present results demonstrated that miR-16 expression in HeLa cells increased when the cells were arrested during S-phase under methyl methanesulfate （MMS） treatment. This further resulted in downregulation of a target protein CDC25A, whereas miR-16 expres- sion did not increase when HeLa ceils were arrested during the MMS-treated G0/G1 or G2/M phase. Furthermore, when HeLa cells were arrested during S-phase with hydroxyurea treatment, miR-16 expression did not increase. These results suggest that expres- sion levels of microRNAs in mammalian cells are delicately regulated under variable cellular conditions.