中文摘要：

在 adipocytes 的类脂化合物微滴用作动物的主要长期的精力存储仓库。正在增加类脂化合物微滴不仅仅是一个静态的中立类脂化合物存储地点，而是事实上有活力、多功能的细胞器的识别。在结构上，类脂化合物微滴由嵌入在或跳了到 phospholipid 层的 phospholipid 单层和蛋白质包围的一个中立类脂化合物核心组成。类脂化合物微滴的表面上的蛋白质对微滴结构和动力学关键。理解类脂化合物有大中央类脂化合物微滴的主要 adipocyte 的联系微滴的 proteome，从 C57BL/6 老鼠的白脂肪质的织物的类脂化合物微滴被孤立。，和由结合的液体层析双人脚踏车团 spectrometry。以前包括 73 的 193 蛋白质的一个总数 unreported 蛋白质被识别。而且，编码同位素的亲密关系标签(ICAT ) 被用来比较类脂化合物的差别在瘦的正常和高脂肪的导致食谱的肥胖的 C57BL/6 老鼠之间的联系微滴的 proteomes。23，蛋白质由 ICAT 分析确定了， 3 蛋白质是起来调整的， 4 蛋白质在从肥胖的老鼠的脂肪质的织物的类脂化合物微滴是下面调整的。重要地，类脂化合物微滴， perilipin A 和 vimentin 的二结构的蛋白质，极大地从肥胖的老鼠在脂肪质的织物的类脂化合物微滴被减少，为类脂化合物的含有的减少的蛋白质机械微滴稳定性。

英文摘要：

Lipid droplets in adipocytes serve as the principal long- term energy storage depot of animals. There is increasing recognition that lipid droplets are not merely a static neutral lipid storage site, but in fact dynamic and multi- functional organeiles. Structurally, lipid droplet consists of a neutral lipid core surrounded by a phospholipid mono- layer and proteins embedded in or bound to the phospho- lipid layer. Proteins on the surface of lipid droplets are crucial to droplet structure and dynamics. To understand the lipid droplet-associated proteome of primary adipocyte with a large central lipid droplet, lipid droplets of white adipose tissue from C57BL/6 mice were isolated. And the proteins were extracted and analyzed by liquid chromatog- raphy coupled with tandem mass spectrometry. A total of 193 proteins including 73 previously unreported proteins were identified. Furthermore, the isotope-coded affinity tags （ICAT） was used to compare the difference of lipid droplet-associated proteomes between the normal lean and the high-fat diet-induced obese C57BL/6 mice. Of 23 proteins quantified by ICAT analysis, 3 proteins were up- regulated and 4 proteins were down-regulated in the lipid droplets of adipose tissue from the obese mice. Importantly, two structural proteins of lipid droplets, perilipin A and vimentin, were greatly reduced in the lipid droplets of the adipose tissue from the obese mice, implicat- ing reduced protein machinery for lipid droplet stability.