中文摘要：

目的建立高效液相色谱法测定人体血浆中羟基红花黄色素A浓度的方法。方法血浆中加入核黄素作内标物，经6％高氯酸沉淀蛋白，取上清液进行HPLC—UV测定。色谱柱为Shim—packVP—ODS C18（150mm×4．6mm，5μm），流动相：乙腈-0．02mol·L^-1磷酸二氢钾溶液（磷酸调节pH至3．5），采用梯度洗脱进行分析，检测波长为403nm，流速：0．8mL／min，室温。结果羟基红花黄色素A的线性范围为0．04—20mg·L^-1（r^2=0．9993），最低定量限为0．04mg·L^-1，平均批内、批间精密度的RSD分别为3．9％、10．6％，平均提取回收率为88．6％。结论本方法简便、快速、准确，适用于羟基红花黄色素A人体临床药动学研究。

英文摘要：

Aim To develop an HPLC method for the determination of hydroxysafflor yellow A in human plasma Methods Riboflavin was used as the internal standard （IS）. The sample was prepared by protein precipitation with 6% perchloric acid. The analytes were separated on a Shim-pack VP-ODS C18 column with a gradient elution system composed of acetonitrile and 0.02mol·L^-1 potassium dihydrogen phosphate solutions （ adjusted to pH 3.5 with phosphoric acid）. The wavelength of UV detection was set at 403nm. The flow rate was 0.8mL/min. Results A linear calibration curve was obtained over the concentrations ranging from 0.04mg·L^-1 to 20mg·L^-1 （ r 2 =0. 999 3 ）. The lower limit of quantification was established at 0.04mg·L^-1. The average intra-batch and inter-batch precisions were 3.9% and 10.6%, respectively. The average recovery was 88.6%. Conclusion This method is rapid, convenient and successfully applied to pharmacokinetic studies of HSYA in human plasma for the first time.