中文摘要：

目的比较红花当归单煎共煎液中有效成分羟基红花黄色素A和阿魏酸含量的变化，初步探索2味中药配伍的变化规律。方法采用RP—HPLC，色谱柱为Shim—packVP—ODSC18（4．6mm×150mm，5μm），流动相为甲醇-0．02mol·L^-1磷酸二氢钾缓冲液（磷酸调节pH至3．5），采用梯度洗脱进行分析；检测波长为230nm，流速为1．0mL·min^-1。结果羟基红花黄色素A回归方程Y=22821ρ-4105．1，r=0．9999，在2．5—50mg·L^-1内线性相关良好，平均加样回收率为100．1％，RSD为2．2％；阿魏酸回归方程Y：70219ρ-2573．9，r=0．9999，在0．5～10mg·L^-1内线性相关良好，平均加样回收率为101．4％，RSD为3．0％。结论红花当归共煎液中羟基红花黄色素A和阿魏酸含量比各单煎液中含量高。共煎更有助于有效成分的提取。本方法简便、快速、准确，适用于同时测定羟基红花黄色素A和阿魏酸的含量。

英文摘要：

OBJECTIVE To compare the content variation of hydroxysafflor yellow A and ferulic acid between single and mixed decoction of Safflower and Angelica. METHODS A RP-HPLC was performed on a Shim-pack VP-ODS C18 column（4.6 mm × 150 mm, 5 μm）with a gradient elution system composed of methanol and 0. 02 mol · L ^-1 potassium dihydrogen phosphate solutions （ adjusted to pH 3.0 with phosphoric acid）. The wavelength of UV detection was set at 230 nm. The flow rate was 1.0 mL · min^-1. RESULTS The calibration curve of hydroxysafflor yellow A was linear within the range of 2.5 - 50 mg ·L ^-1 （ Y = 22 82 1ρ - 4 105. 1, r =0. 999 9）. The mean absolute recovery was 100. 1% and RSD was 2. 2%. The curve of ferulic acid was linear within the range of 0. 5 - 10 mg · L^-1 （ Y =70 219ρ -2 573.9 ,r =0. 999 9）. The mean absolute recovery was 101.4% and RSD was 3.0%. CONCLUSION The content of hydroxysafflor yellow A and ferulic acid in mixed decoction is higher than the single decoction of Safflower and Angelica. The mixed decoction is favorable for the extraction of hydroxysafflor yellow A and ferulic acid. The method is convenient, rapid, accurate and successfully applied to detect hydroxysafflor yellow A and fendic acid simultaneously.