中文摘要：

通过沙培实验和采用差速离心技术,研究了钒（V）在枸杞幼苗中的积累、转运和亚细胞分布特征。结果表明：V在枸杞不同器官中的含量和分配比例为根〉茎〉叶,随着V处理浓度的增加,枸杞幼苗根中V含量和分配比例显著增大,在V处理浓度为20mg·L^-1时,根中V含量达19.80μg·g-1,占总量的60.1%。枸杞幼苗在V浓度为2、5mg·L^-1时,根富集系数BCF〉1,转运系数TF〈1,表明枸杞植物对V有较强的富集能力,但转运能力较低,其积累的V主要富集在枸杞植株根部,可减轻过量V对茎叶的毒害。V在枸杞叶片中的亚细胞分布规律为F1（细胞壁）〉F4（可溶性组分）〉F2（细胞核）〉F3（线粒体和叶绿体）。V在细胞壁和可溶性组分中所占的比例分别为28.3%-52.4%和4.1%-45.2%,表明细胞壁是V在枸杞叶片细胞中的重要分布位点。随着V处理浓度的增加,V在细胞壁中的分配比例减少,而可溶性组分的分配比例相应增加,细胞核、线粒体和叶绿体的分配比例显著下降。细胞壁和细胞质可溶性组分的相互协调,对植物自身解毒重金属起着重要作用,从而增强枸杞植物对重金属V的耐性。

英文摘要：

Accumulation, translocation and subcellular distribution of Vanadium（V） in Lycium barbarum seedling was investigated with sand culture by the differential centrifugation. Results showed that allocation proportions and concentrations of V in different organs of Lycium barbarum seedling were in sequence as follows：rootpetioleleaf. With V press level increasing, concentrations and allocation proportions of V in root increased significantly, and reached to the maximum,19.80 μg·g -1 and 60.1% respectively when V press level was 20 mg·L-1 . The bioconcentration factor（BCF）and the translocation factor（TF）for V was more than 1 and less than 1 respectively when V press level was 2 mg·L-1 or 5 mg·L-1 . It suggested that there is a strong enrichment and low translocation in Lycium barbarum seedling, while most V is accumulated in roots, which can reduce the toxicity of V to petioles and leaves. The subcellular distribution level of V in the leaves of Lycium barbarum seedling was in sequence as follows：F1（cell wall and residue）F4（soluble fraction）F2（nucleus）F3（mito-chondrion and chloroplasts）. The allocation proportions of V in F1 and F4 were 28.3%～52.4% and 4.1%～45.2% respectively, indicating that cell wall is the main V-biding site in leaves of Lycium barbarum seedling. With V press level increasing, the allocation proportions of V decreased in cell wall, nucleus, mitochondrion and chloroplasts, but increased in soluble fraction, suggesting that intercoordination between cell wall and soluble fraction may benefit the tolerance of Lycium barbarum seedling to V.