中文摘要：

背景与目的通过检测聚集素(Clusterin,CLU)在耐药非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞系Anip973/Navelbine(Anip973/NVB)中的表达,并以Anip973作为对照。证实CLU与NSCLC细胞系对NVB的耐药相关。同时,检测CLU、p53(突变型)、Bax在正常肺组织、肺癌组织中的表达,探讨其与肺癌发生发展的关系。方法采用WesternBlot法从蛋白水平比较CLU在Anip973、Anip973/NVB中的差异表达,并通过流式细胞仪检测CLU阳性表达率、CLU与细胞周期、CLU与p53、Bax之间的关系。结果CLU与p53在NSCLC耐药细胞系Anip973/NVB中的表达均显著高于Anip973(P<0.01),Bax在NSCLC耐药细胞系Anip973/NVB中的表达于Anip973相比无统计学差异(P>0.05)。进入G0-G1期的Anip973/NVBNSCLC耐药细胞明显多于非耐药细胞,而进入S期G2-M期的Anip973/NVBNSCLC耐药细胞明显少于非耐药细胞。结论CLU与p53在NSCLC耐药细胞系中呈高表达,表...

英文摘要：

Background and objective To detect clusterin expression in Anip973/Navelbine(Anip973/NVB) and wild type Anip973,the last one as a control,so as to conform that clusterin be association with the resistance to NVB of NSCLC.We detect the expression of clusterin,p53 and Bax in normal lung tissues and cancer tissues so that to investigate its relationship with lung cancer formation and development.Methods Western blot was used to compare the differences in protein levels of clusterin in Anip973/NVB and wild type...