中文摘要：

目的利用流式细胞技术检测结核分枝杆菌国际标准强毒株H37Rv株和卡介苗菌株（BCG）分别感染巨噬细胞的凋亡率及其时相性变化。方法分别用结核分枝杆菌H37Rv株和BCG菌株感染巨噬细胞RAW264.7细胞株,于感染后1、3、6及12h,用流式细胞技术检测各组感染巨噬细胞的凋亡率,并分析其时相性变化。结果结核分枝杆菌H37Rv株和BCG菌株感染均使巨噬细胞凋亡率升高,其中在感染后1h和12h凋亡率升高更显著,两组比较凋亡率差异有统计学意义（P〈0.05）。结论结核分枝杆菌感染导致巨噬细胞凋亡,凋亡率与菌株毒力强弱相关,毒力弱的结核分枝杆菌感染早期巨噬细胞升高更显著。

英文摘要：

Objective To compare the rate of apoptosis of macrophages infected with H37Rv and BCG using flow cytometry.Methods RAW264.7 cells were infected with H37Rv and BCG strains.Flow cytometry was used to detect the rate of macrophage apoptosis 1 h,3 h,6 h,and 12 h after infection.Changes over time in the rate of apoptosis of infected macrophages were analyzed.Results H37Rv and BCG increased the rate of macrophage apoptosis.Apoptosis increased markedly 1h and 12 h after infection.There were significant differences in the rate of apoptosis of macrophages infected with BCG and macrophages infected with H37Rv（P〈0.05）.Conclusion M.tuberculosis induced the apoptosis of macrophages.The rate of apoptosis correlated with the virulence of the strain of M.tuberculosis.Apoptosis of macrophages infected with the less virulent M.tuberculosis soon increased markedly.