目的探讨甘露聚糖结合凝集素(mannan-binding lectin,MBL)基因的多态性是否与新疆维吾尔族人群结核病发病相关。方法通过使用引物序列特异性PCR(SSP-PCR)的方法分别对226例新疆维吾尔族活动性肺结核患者(简称病例组)及231例有结核分枝杆菌接触史的新疆同民族健康者(简称对照组)进行MBL基因的A/B多态性位点进行基因分型,统计学分析采用病例对照分析研究不同基因型与新疆维吾尔族结核病易感性的关系。结果在病例组中,MBL基因A/B位点AA基因型106例(占46.90%),AB基因型106例(占46.90%),BB基因型14例(占6.20%);对照组AA基因型则为146例(占63.20%),AB基因型80例(占34.63%),BB基因型5例(占2.17%)。病例组中MBL-AB突变基因频率显著高于健康对照组,两组的突变基因频率分别为6.20%、2.17%,差异有统计学意义(χ^25.224,Pc〈0.05)。结论新疆维吾尔族人群中MBL基因AA基因型可能为结核病的保护性因素,BB基因型可能为结核病发病的危险性因素。新疆维吾尔族人群中MBL基因A/B位点多态性与结核病易感性有明显相关性。
Objective To study the relationship between the polymorphism of the MBL gene and the susceptibility of tuberculosis(TB) in Xinjiang Uighur population.Methods The polymorphism of the MBL-A/B gene was analyzed by PCR-sequence specific primer(SSP) in 226 patients with pulmonary TB and 231 healthy controls who had a history of exposure to M.tuberculosis in Xinjiang Uighur population.The relationship between MBLgenotypes and the susceptibility of pulmonary TB was studied in accordance to the case-control study,and cases were grouped according to the genotypes.Results In the case group,AA,AB and BB genotypes at MBL gene A/B site were 106 cases(46.90%),106 cases(46.90%) and 14 cases(6.20%),respectively.In the control group,AA,AB and BB genotypes were 146 cases(63.20%),80 cases(34.63%) and 5 cases(2.17%),respectively.The frequency of BB genotypes at MBL gene A/B site in the case group was significantly higher than that in the healthy control group(6.20% vs 2.17%)(χ^25.224,Pc〈0.05).Conclusion AA genotype of MBL gene may be protective factor for TB,while BB genotype may be risk factor for TB in Xinjiang Uighur population.