中文摘要：

选择FITC和罗丹明类荧光分子Alexa Fluor（R）514作为荧光对，通过酶-化学方法合成了一种潜在的FRET多肽．首先，在OGT催化下，将N-叠氮乙酰葡糖胺（GlcNAz）通过O-糖苷键连接到FITC标记的底物八肽（YAVVPVSK）的丝氨酸羟基上；然后，将炔基标记的Alexa Fluor（R）514通过点击化学反应连接到GlcNAz上，得到目标化合物，并通过质谱进行了表征．

英文摘要：

A chimerical peptide with a fluorescence resonance energy transfer （FRET） pair, Alexa Fluor（R） 514 and FITC-tagged, was synthesized via enzyme-chemical method. First- ly, an octapeptide （YAVVPVSK） was labeled with fluorescein （FITC） at C- or N-terminus, which was linked with GlcNAz via sOGT mediated glycosyl-transferring reaction. Then Fluo- rescein Alexa Fluor（R） 514 was linked to the azide group via click reaction. The resultant molecule was identified via MALDI-TOF-MS.