中文摘要：

O-GlcNAc是一种广泛存在于蛋白质丝/苏氨酸残基上的动态、可逆的蛋白翻译后修饰，它广泛分布在细胞浆和细胞核中，参与调节多种细胞途径。研究表明蛋白的O-GlcNAc糖基化与神经退行性疾病、糖尿病和癌症等疾病相关。在体内，O-GlcNAc动态修饰由N-乙酰氨基葡萄糖转移酶（OGT）和N-乙酰氨基葡萄糖苷酶（OGA）协同完成。近年来，OGT逐渐成为糖生物学领域的研究热点，在其结构、作用机制及晶体学方面取得了快速发展。

英文摘要：

N-acetylglucosamine （O-GlcNAc） modification on protein serines/threonines is a dynamic, inducible and abundant post-translational modification, which is found on numerous cytoplasm and nucleus proteins, regulating many cellular processes. It has been demonstrated that O-GlcNAc plays important roles in some human diseases, such as diabetes and neurodegenerative diseases. O-GlcNAcylation is regulated by O-GlcNAc transferase （OGT） and O-GlcNAcase （OGA） in vivo. The past decades has seen a mass of research on OGT, include studies on the protein structure and the mechanism linked to many important diseases. Recently, OGT is becoming the spotlight in glyco-biological research.