中文摘要：

目的：研究大鼠脊神经结扎（spinal nerve ligation,SNL）神经痛模型不同时相背根神经节（dorsal root ganglion,DRG）TRPV1的活化形式.方法：大鼠随机分为正常（D 3）组、模型（D 3）组、正常（D 15）组与模型（D 15）组.采用SNL方法制作大鼠神经痛模型.检测造模后第三天（D 3）与第15天（D 15）大鼠术侧缩足阈值（paw withdrawal threshold,PWT）,L4～6 DRG TRPV1表达与磷酸化水平.结果：SNL模型大鼠早期即出现痛觉敏化现象,直至实验结束.D3、D15术侧PWT显著下降（P＜0.001）.D3 L4～6DRG TRPV1表达均下降（p＜0.05,P＜0.01,P＜ 0.01）;L5 DRG TRPV1磷酸化水平上升（P＜ 0.001）,L4、L6 DRG TRPV1磷酸化水平下降（P＜0.01,P＜ 0.05）.D 15 L5 DRG TRPV1表达水平下降（P＜0.01）,L4、L6 DRG TRPV1表达水平上升（P＜0.05）;L4～6 DRG TRPV1磷酸化水平均下降（P＜0.01,P＜0.001）.结论：SNL神经痛的发生、维持与DRG TRPV1的活化有关.SNL神经痛的早期,TRPV1的活化与损伤DRG TRPV1磷酸化水平升高有关;SNL神经痛的维持阶段,TRPV1的活化与邻近未损伤DRG TRPV1表达增强有关.

英文摘要：

Objective：To investigate the changes in the activation modes of DRG TRPV1 at different periods of SNL-induced neuropathic pain in rats.Methods：Rats were randomly divided into normal group （day 3）,SNL group （day 3）,normal group （day 15）,and SNL group （day 15）.Rat neuropathic pain model was made by ligating right L5 spinal nerve.In D 3 and D 15 after SNL,paw withdrawal threshold （PWT）,L4-6 DRG TRPV 1 expression and phosphorylation level were measured.Results：Rats in SNL group developed spontaneous pain and showed a significant reduction in PWT in D 3 and D 15 （P 〈 0.001）.In D 3,L4-6 DRG TRPV1 expression reduced （P 〈 0.05,P 〈 0.01）.L5 DRG p-TRPV1 level increased （P 〈 0.001）,while L4 and L6 DRG p-TRPV1 level reduced （P 〈 0.01,P 〈 0.05）.In D 15,L5 DRG TRPV1 expression reduced （P 〈 0.01）,while L4 and L6 DRG TRPV1 expression increased （P 〈 0.05）.L4-6 DRG p-TRPV1 reduced （P 〈 0.01,P 〈 0.001）.Conclusion：The induction and maintainence of SNL-induced neuropathic pain were associated with the activation of DRG TRPV1.In early stage of SNL-induced neuropathic pain,TRPV1 activation was associated with up-regulation of p-TRPV1 in injured DRG,while in the maintenance of SNL-induced neuropathic pain,TRPV1 activation was related to the upregulation of TRPV1 expression in adjacent uninjured DRGs.