中文摘要：

目的：研究2和100Hz电针对2型糖尿病神经痛（DNP）大鼠的镇痛作用，以及对DNP大鼠背根神经节（DRG）上P2X3受体和降钙素相关基因肽（CGRP）表达的影响。创新点：本研究首次选用2型DNP大鼠模型来研究电针治疗DNP的优势频率。研究证明2Hz电针对2型DNP大鼠的镇痛作用以及对DRG上高表达的P2X3抑制作用优于100Hz，为电针治疗DNP及其频率选择提供科学依据和阐释。方法：将雄性SD大鼠分为对照组（腹腔注射柠檬酸钠缓冲液）和模型组（高脂高糖饲养联合腹腔注射35mg／kg链脲佐菌素（STZ））。模型组根据采用不同的电针频率分为以下三组：DNP组、DNP＋2Hz电针组、DNP＋100Hz电针组。采用足底测试仪检测大鼠足跖热痛阈（PWL），用酶联免疫吸附测定（ELIsA）试剂盒检测大鼠空腹胰岛素（FTNs）含量，用免疫荧光法检测DRG上的P2X3受体和CGRP的表达水平。结论：2和100Hz电针对2型DNP均有明显的镇痛作用，且2Hz电针对2型DNP的镇痛作用优于100Hz电针（图2）。免疫荧光实验结果显示，2和100Hz电针均能明显下调DRG中P2X3受体和CGRP的高表达（图3和4），且2Hz屯针对DRG中P2X3受体的抑制作用优于100Hz电针（图3）。综上所述，2Hz电针是治疗2型DNP较为理想的治疗手段。

英文摘要：

Objective： To investigate the analgesic effects of electroacupuncture （EA） at 2 and 100 Hz on type 2 diabetic neuropathic pain （DNP） and on the expressions of the P2X3 receptor and calcitonin gene-related peptide （CGRP） in the dorsal root ganglion （DRG）. Methods： Rat type 2 DNP was induced by a high calorie and high sugar diet fed for 7 weeks, plus a single intraperitoneal injection of streptozotocin （STZ） after 5 weeks. EA at 2 and 100 Hz was carried out once every day after 7 weeks for 7 consecutive days. Body weight, serum fasting insulin （FINS）, fasting blood glucose （FBG）, insulin sensitivity index （ISI）, and paw withdrawal latency （PWL） were measured. The expressions of L4-L6 DRG P2X3 receptors and CGRP were assessed by immunofluorescence. Results： Type 2 DNP was successfully induced as shown by the increased body weight, FINS, and FBG, as well as the reduced ISI and PWL. Expressions of P2X3 receptors and CGRP in L4-L6 DRGs increased. EA at both 2 and 100 Hz relieved type 2 DNP, but the analgesic effect of EA was stronger at 2 Hz. P2X3 receptor expression decreased in L4-L6 DRGs following EA at 2 Hz and in L5 and L6 DRGs following EA at 100 Hz. EA at both 2 and 100 Hz down-regulated CGRP overexpression in L4-L6 DRGs. Conclusions： These findings indicate that EA at 2 Hz is a good option for the management of type 2 DNP. The EA effect may be related to its down-regulation of the overexpressions of the DRG P2X3 receptors and CGRP in this condition.