中文摘要：

电化学葡萄糖传感器检测血浆中的葡萄糖时,血浆中的电活性物质-对乙酰氨基酚会严重干扰检测结果。本研究以对乙酰氨基酚（ACP）为电子转移媒介体,研究了葡萄糖氧化酶（GOD）修饰电极的电化学行为,并探讨了不同pH条件下该修饰电极催化葡萄糖氧化的能力。研究发现ACP能有效地促进酶活性中心与电极间的电子转移,且Nafion涂覆的GOD修饰电极能有效排除尿酸（UA）和抗坏血酸（AA）的干扰。实验结果表明Nafion/GOD电极的氧化电流与葡萄糖浓度在0.6～15mmol/L范围内呈良好的线性关系,且对葡萄糖的检测限为59μmol/L。

英文摘要：

Acetaminophen,one of the most electroactive compounds in plasma,can interfere the electrochemical detection of glucose.Acetaminophen is used as a mediator for the first time in this research,the electrochemical behaviors and the ability of the glucose oxidase（GOD）modified electrode for catalytic oxidation of glucose at various pH were investigated.The results demonstrated that acetaminophen can promote the electron transfer rate from the active sites of GOD to the electrode surface.And the Nafion film was coated on the modified electrode to eliminate the interference such as ascorbic acid,uric acid during the oxidation of glucose.The oxidation peak current at the modified electrode exhibited a linear relationship with the glucose concentration in the range of 0.6-15mmol/L with a detection limit of 59nmol/L.