中文摘要：

番茄果实的成熟是由多基因精细调控的一个过程．利用破色期番茄果实，根据复性动力学原理在mRNA水平进行均一化操作使高丰度和低丰度的mRNA丰度接近．然后把均一化之后mRNA反转录得到cDNA，再与基因沉默载体pTRV重组，最后把构建好的载体通过电转化的方法转入到GV3101农杆菌中，从而建立起破色期番茄果实均一化cDNA沉默文库．通过番茄果实中病毒诱导基因沉默技术，对cDNA沉默文库进行初步筛选，从而确定功能基因筛选模型．在模型建立阶段，以番茄红素合成途径相关的PDS基因作为内标基因，在100个混合农杆菌样中，成功筛选到了PDS基因．

英文摘要：

The ripeness of tomato is regulated by multi-genes. Transcripts of mRNA extracted from tomato at breaker stage were normalized, and cDNA with pTRV was constructed after reverse transcription. In order to construct a normalized cDNA silencing library, a novel procedure was adopted to reduce the abundant copy number and increase the rare copy number. Then the normalized cDNA was put into pTRV vector to construct a cDNA library and this cDNA library was screened with the technology of virus induced gene silencing. PDS, which was related about synthesis of lycopene, was used as marker gene to establish the model of screening. Among one hundred mixed agrobateria, PDS was successfully screened in the first stage of model establishment.