中文摘要：

果实组织巾往往富含多糖和多酚，由此导致了果实组织比其他植物组织总RNA的提取要困难的多。本研究以番茄为模式材料，探索自制TRIZOL试剂法从富含多糖的果实组织中提取总RNA。以葡萄为模式材料探索PVP改进法提取富含多酚的果实组织的总RNA的方法。经过纯度、得率检测证明所研究的两种新方法能够保证从富含多糖和多酚的果实组织中获得高得率和高质量的总RNA。

英文摘要：

The isolation of large quantities of good-quality RNA from fruit tissues rich in polysaccharide or polyphenol has been a long-standing problem for researchers. In this study, a self-prepared TRIZOL reagent was used to extract total RNA from tomato （rich in polysaccharide）. Also, an improved PVP method was applied to extract total RNA from grape （rich in polyphenol）. Compared to guanidinium thiocyanate method, the above methods resulted in higher purity and yield of total RNA from tomato and grape, respectively.