中文摘要：

目的观察天麻钩藤饮对帕金森病（PD）大鼠神经行为学及氧化应激的影响。方法采用6羟基多巴胺（6-OHDA）注射于脑右侧黑质造成单侧PD损毁模型,并用中药天麻钩藤饮（浓度为5.13g/ml）进行治疗,共给药14d。同时设立正常对照组、假手术组,观察各组大鼠神经行为学的变化,同时运用化学比色法测定大鼠中脑黑质、纹状体部位活性氧、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、丙二醛（MDA）的活性。结果神经行为学方面,模型组大鼠治疗前后旋转圈数无显著差异;天麻钩藤饮组大鼠旋转圈数较模型组显著减少（P〈0.05）,且治疗前后比较差异显著（P〈0.05）;模型组活性氧、MDA明显升高,GSH、GSH-Px、SOD明显降低,与正常对照组、假手术组比较,差异显著（P〈0.05或P〈0.01）;而天麻钩藤饮对其均有明显的改善作用（P〈0.05）。结论天麻钩藤饮可以明显改善PD大鼠的神经行为学变化,并可以提高机体的抗氧化和清除自由基的能力。

英文摘要：

Objective To observe effect of tianma gouteng drink on the behavioural and oxidation stress response of Parkinson＇ s disease （PD） rat. Methods Rats were divided into normal control, sham operation, model, and treatment groups （6 rats in each group）. PD rats were produced by injection of 6-OHDA stereotaxically into the right side of the mesencephic ventral tegmental area （VTA） and sub- stantia nigra pars eompacta （SNc） and treated with tianma gouteng drink （5. 13 g/ml） for 14 days. Behavioural changes and the activity of reactive oxygen species （ ROS）, glutathione （ GSH）, glutathione peroxidase （ GSH-Px）, superoxidase dismutase （ SOD）, malonaldehyde （MDA） of the substantia nigra and corpus striatum of the PD rats were observed. Results There was no significant difference in rotation be- haviour between before and after treatment in model group （ P 〉 0. 05 ）. Compared to model group, the rotation behaviour was significantly decreased in treatment group, and there was significant difference between before and after treatment in treatment group （P 〈 0. 05）. Com- pared with the normal control and sham operation groups, ROS and MDA contents were obviously increased and GSH, GSH-Px and SOD con- tents were obviously reduced in model group （P 〈 0.05 ）. However, the above indicators were improved in treatment group （P ~ 0. 05 ）. Conclusions Tianma gouteng drink can significantly improve the neurological behavior, and enhance anti-oxidation ability and eliminate free radicals of PD rats.