中文摘要：

目的：研究脊髓肿瘤坏死因子-alpha（TNF-α）在急性切割损伤导致的痛觉过敏中的作用；方法：在异氟醚麻醉下纵行切割大鼠后足，在手术不同时段观察大鼠切割足50％缩足阈值，并取腰髓节段采用实时定量RT-PCR观察TNF-α的mRNA水平。另外一组实验，鞘内给予可溶性TNF受体蛋白（依那西普，Etanercept）观察其切割后足大鼠的痛觉行为学改变。结果：大鼠后足切割导致其同侧腰段脊髓TNF-α基因水平上调，鞘内注射依那西普显著抑制切割损伤引起的机械性痛觉过敏。结论：脊髓内TNF-α参与了切割损伤导致的痛觉过敏，抑制上调的TNF-有可能成为临床上治疗术后疼痛的新方法。

英文摘要：

Objective： To investigate the pathogenic role of tumor necrosis factor-alpha （TNF-α） in incision-induced mechanical hypersensitivity in the rats. Methods： A longitudinal incision was made in one plantar hind paw of isoflurane-anesthetized rats. Mechanical hypersensitivity was assessed by withdrawal thresholds from mechanical stimuli of von Frey filaments. The gene expression of TNF-α in the spinal cord was determined by Real-time PCR. Intrathecal injection of TNF- soluble receptor, Enatercept was performed to modulate the spinal TNF-α after surgical incision. Results： After hind-paw incision, the TNF-α mRNA level was upregulated in the ipsilateral lumbar spinal cord. Intrathecal injection of Enatercept dramatically inhibited the surgical incision-evoked pain hypersensitivity. Conclusion： The present study showed that the upregulated TNF-α in the spinal cord contributed to the pain hypersensitivity after surgical incision.