中文摘要：

目的：观察脑源性神经营养因子（BDNF）对大鼠后足切割疼痛的影响。方法：采用纵行切割大鼠后足作为疼痛模型,运用免疫组织化学与免疫荧光双标记方法,观察大鼠后足切割后不同时间点（1-72hr）BDNF在相应节段背根神经节与脊髓内表达的变化。腹腔或鞘内注射BDNF抗体中和内源性BDNF后,以Von Frey尼龙纤维刺激后足行机械痛敏评价。结果：大鼠后足切割后1-24hr内,BDNF在切割侧L42-L5脊髓后角表达明增加,BDNF主要位于后角神经元内与神经末梢,星形胶质细胞与小胶质细胞内未见明显表达;在L42-L5背根神经节,BNDF免疫阳性细胞百分比在切割后1-24hr内也明显增加,增加的主要为大直经神经元;鞘内给予BDNF抗体可明显增加大鼠后足切割后的缩足阈值,而腹腔给予BDNF抗体对大鼠的缩足阈值影响较小。结论：BDNF参与了大鼠后足切割后机械痛敏的过程。

英文摘要：

Objective：To investigate the role of brain derived neutrophic factor in the surgical incision-evoked pain hypersensitivity.Method：Incisional pain rat model was established by a longitudinal incision in right plantar hind paw of isoflurane-anesthetized rats.Dorsal root ganglias（DRG） and spinal cords were removed at various postoperative times（1-72 h）.Expression pattern of BDNF was determined by immunohistochemistry and double-labeling immunofluorescence.BDNF monoclonal antibody was administered intrathecally（IT） or intraperitoneal（IP） to modulate the spinal BDNF or peripheral BDNF after incision.Result：The expression of BDNF in the ipsilateral lumbar DRG and spinal cord was increased after hind-paw incision.The upregulated spinal BDNF was mainly localized in the neurons but not microglia or astrocytes.IT injection of BDNF antibody greatly inhibited the mechanical allodynia induced by incision whereas IP administration had only marginal effect.Conclusion：The present study showed that incision induced the upregulation of BDNF in the DRG and spinal cord,and the upregulated BDNF contributed to the pain hypersentivity induced by surgical incision.