欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的鉴定骨髓瘤抗原Sp17的HLA-A＊0201限制性CTL表位。方法采用超基序和量化基序法预测Sp17的HLA-A＊0201限制性CTL表位；用T2细胞亲和力实验和稳定性实验对该表位进行初步鉴定。结果超基序和量化基序法预测得出Sp17抗原的4个CTL表位（LLEGLTREI（19-27）、ILREQPDNI（27-35）、SLLEKREKT（45-53）、KEKEEVAAV（111-119）），亲和力实验结果表明LLEGLTREI（19-27）、ILREQPDNI（27-35）、SLLEKREKT（45-53）有较高亲和力，而KEKEEVAAV（111-119）肽亲和力较低；稳定性实验表明ILREQPDNI（27-35）、SLLEKREKT（45-53）与HLA-A＊0201分子结合稳定性较好。结论ILREQPDNI（27-35）、SLLEKREKT（45-53）有可能是骨髓瘤抗原Sa17 HLA-A＊0201限制件CTL表位。
英文摘要：
Objective To identify HLA-A ＊ 0201-restricted CTL epitopes from multiple myeloma tumor antigen Sp17. Methods The HLA-A ＊ 0201-restricted CTL epitopes were predicted by supermotif combined with quantitative motif, and then evaluated by T2 binding assay and MHC stabilization assay. Results Four candidate nonapeptides were predicted by supermotif combined with quantitative motif. T2 binding assay suggested that among the four peptides. LLEGLTREI（19-27）. ILREQPDNI（27-35）, and SLLEKREKT（45-53） had higher affinity for HLA-A ＊ 0201 molecular as compared to SLLEKREKT（45-53）. Peptides ILREQPDNI（27-35） and SLLEKREKT（45-53） could bind stably with HLA-A ＊ 0201 molecular. Conclusion Peptides ILREQPDNI（27-35） and SLLEKREKT（45-53） may be the HLA-A ＊ 0201-restricted CTL epitopes of Sp17.
同期刊论文项目
同项目期刊论文
Inhibition of severe acute respiratory syndrome-associated coronavirus infection by equine neutraliz
Cross-presentation of phage particle antigen in MHC class II and endoplasmic reticulum marker-positi
Triptolide inhibits B7-H1 expression on proinflammatory factor activated renal tubular epithelial ce
Endothelial stroma programs hematopoietic stem cells to differentiate into regulatory dendritic cell
Comprehensive antibody epitope mapping of the nucleocapsid protein of severe acute respiratory syndr
Peptide-dependent inhibition of alloreactive T-cell response by soluble divalent HLA-A2/IgG molecule
Evaluation of the safety, immunogenicity and pharmacokinetics of equine anti-SARS-CoV F(ab')(2) in m
Soluble mouse B7-H3 down-regulates dendritic cell stimulatory capacity to allogenic T cell prolifera
Gene delivery of indoleamine 2,3-dioxygenase prolongs cardiac allograft survival by shaping the type
Protective immunity elicited by a divalent DNA vaccine encoding both the L7/L12 and Omp16 genes of B
An altered peptide ligand for naive cytotoxic T lymphocyte epitope of TRP-2((180-188)) enhanced immu
Identification of amyloid fibril-forming segments based on structure and residue-based statistical p
Protection of mammalian cells from severe acute respiratory syndrome coronavirus infection by equine
Steady state dendritic cells with forced IDO expression induce skin allograft tolerance by upregulat
Osteopontin expression in human decidua is associated with decidual natural killer cells recruitment
IL-10 gene modified dendritic cells inhibit T helper type 1-mediated alloimmune responses and promot
Identification of a common HLA-A*0201-restricted epitope among SSX family members by mimicking alter
RAET1E2, a Soluble Isoform of the UL16-binding Protein RAET1E produced by tumor cells, inhibits NKG2
Sinomenine promotes differentiation but impedes maturation and co-stimulatory molecule expression of
A novel mimovirus vaccine containing survivin epitope with adjuvant IL-15 induces long-lasting cellu
Fas signal links innate and adaptive immunity by promoting dendritic cell secretion of CC and CXC ch
Experimental studies on the gene therapy of CACX by mimoretrovirus- delivered siRNA targeting POKEMO
期刊信息
位置：