中文摘要：

本文研究了复方海鳗鱼皮明胶（Fufang Pike Eel Skin Gelatin,FEG）对大鼠缺铁性贫血的改善作用,旨在找到一种新型的复方补血品。本文采用热提法制备海鳗鱼皮明胶,配合当归、白芍、川芎、黄芪、炒山楂、制首乌和熟地黄七种中药材熬制得到FEG。采用传统的低铁饮食辅助尾静脉放血的方法建立缺铁性贫血（Iron Deficiency Anemia,IDA）模型,将IDA大鼠随机分成模型组、FEG和复方阿胶高低剂量组。灌胃30 d,通过测定大鼠的血红蛋白（Hb）、红细胞数（RBC）、血清中的铁（SI）、铁蛋白（SF）和可溶性转铁蛋白受体（Soluble Transferrin Receptor,sTfR）浓度等指标以及肝脏HE染色和骨髓涂片评价其补血效果。结果表明,FEG高剂量组能够显著提高IDA大鼠的RBC（p〈0.01）,降低网织红细胞数（p〈0.05）、肝脏（p〈0.01）、脾脏（p〈0.05）和肾脏系数（p〈0.01）,缓解骨髓异常增生和肝组织紊乱;使Hb浓度由77.21 g/L提高到175.86 g/L;使SI和SF浓度分别升高了2.9和0.6倍,sTfR浓度降低了19.42%。复方海鳗鱼皮明胶能够有效地改善缺铁性贫血。

英文摘要：

With the aim of developing a new kind of compound hematopoietic health product, the hematopoietic effect of Fufang pike eel skin gelatin（FEG） on iron deficiency anemia in rats was investigated. The pike eel skin gelatin was prepared using a hot water extraction method, and was combined with Angelica sinensis, Radix Paeoniae Alba, Rhizoma Chuanxiong, Astragalus membranaceus, Radix Polygoni Multiflori Preparata, Fructus Crataegi, and Radix Rehmanniae Preparata. A rat model of iron deficiency anemia（IDA） was established by feeding rats with a traditional low-iron diet and bleeding from the caudal vein. Then, the IDA rats were randomly divided into the following five groups（ten rats per group）： model control group, FEG high and low dose groups（FEG-H and FEG-L）, and Fufang donkey-hide gelatin high and low dose groups（PC-H and PC-L）. After 30 d of administration by gavage, the hematopoietic effect was evaluated by measuring the concentrations of hemoglobin（Hb）, serum iron（SI）, ferritin（SF）, and soluble transferrin receptor（sTfR）, and performing red blood cell counts（RBC）, hematoxylin and eosin（H E） staining of liver tissues, and bone marrow smears. The results showed that high dose FEG could significantly improve the value of RBC（p〈0.01）, reduce the reticulocyte count（p〈0.05） and the indices of liver（p〈0.01）, spleen（p〈0.05）, and kidney（p〈0.01）, and alleviate symptoms of bone marrow hyperplasia and liver tissue disorder. Additionally, high dose FEG enhanced Hb content from 77.21 g/L to 175.86 g/L, increased SI and SF concentrations by 2.9 and 0.6 times, respectively, and reduced sTfR concentration by 19.42%. Fufang pike eel skin gelatin could effectively improve the symptoms of IDA.