中文摘要：

为了评价鳕鱼皮胶原蛋白源金属螯合肽,Ala-Gly-Pro-Ala-Gly-Pro-Arg（多肽M）,的胃肠消化耐受性,建立体外模拟胃肠消化模型（SGID）,该模型包括模拟人体中胃消化环境和肠消化环境两个阶段。多肽M的钙螯合率为0.88±0.02μg/mg,铁螯合率为33.67%。胃消化产物S,钙螯合率为0.87±0.03μg/mg,与纯肽相比钙螯合率下降1.14%;铁螯合率为33.72%,与纯肽相比反而上升0.05%。肠消化产物D,钙螯合率为0.85±0.01μg/mg,与纯肽相比下降3.40%;铁螯合率为34.13%,与纯肽相比上升0.46%。利用反相高效液相色谱、质谱和圆二色谱技术,发现经过两个阶段多肽M分子量未发生变化,肽键未发生断裂,构象发生变化,其中无规卷曲减少,β折叠和β转角构象增加。因此,多肽M在体外模拟胃肠消化过程中其多肽链氨基酸组成不变,空间构象发生改变,钙铁离子螯合能力变化不大,体外模拟胃肠消化耐受性高。

英文摘要：

In order to evaluate the gastrointestinal tolerance of a new Alaska pollock skin collagen-derived metal chelating peptide（Ala-Gly-Pro-Ala-Gly-Pro-Arg,peptide M）,an in vitro simulated gastrointestinal digestion（SGID） model was established,and this model included two stages：simulated human gastric digestion and simulated human intestinal digestion.The calcium-chelating activity of peptide M was 0.88 ± 0.02 μg/mg,and its iron-chelating activity was 33.67%.The calcium-chelating activity of gastric digestion product S was 0.87 ± 0.03 μg/mg,a 1.14% decrease compared to the pure peptide.The iron-chelating activity of S was 33.72%,an increase of 0.05% compared to the pure peptide.The calcium-chelating activity of intestinal digestion product D was 0.85 ± 0.01 μg/mg,a 3.40% decrease compared to the pure peptide.And the iron-chelating activity of D was 34.13%,an increase of 0.46% compared to the pure peptide.Reversed-phase high-performance liquid chromatography,full scan mass spectrometry,and circular dichroism showed that after two-stage SGID,M molecule did not show a change in molecular mass or cleavage of peptide bonds,but there were changes in the conformation,including a decrease in the proportion of random coils and increases in the proportions of β-sheets and β-turns.Therefore,during the SGID,the amino acid composition in the peptide was not changed,and the spatial conformation was changed.The calcium- and iron-chelating capabilities were not significantly changed.Peptide M showed a high gastrointestinal tolerance in SGID.