中文摘要：

转移生长因子β（TβRI）信号传导通路参与调节细胞的增殖、分化、凋亡、细胞迁移等一系列细胞过程，与骨代谢疾病的发病机制密切相关．本研究根据荧光共振能量转移（FRET）技术原理，构建包含CFP-TβRI-YFP融合蛋白的TβRI生物传感器，转染293T细胞，观察转染效率．以TGFβ1为诱导剂，激活TGFβ／TβRI信号传导通路，在活细胞生理条件下，动态监测TβRI生物传感器的FRET效应．结果表明，成功构建了TβRI生物传感器，转染细胞效率达50％，在TGFβ1诱导刺激6min后，FRET效率增加并达到最大值，该过程经历9min后，随时间的延长，FRET效率下降．研究结果表明：在活细胞生理条件下，TGFβ1／TβRI信号转导过程存在一定的时间特异性．

英文摘要：

The interaction between transforming growth factor β （TGFβ） and TGFβ receptor I （TβRI） played a critical role of regulation of a variety of osteoporosis-related processes, such as cell proliferation, differentiation, apoptosis and migration. We constructed a TβRI biosensor in the form of a CFP-TβRI- YFP fusion protein. The TβRI biosensor construct was transfected into the 293T cells at about 50% of efficiency. Fluorescence resonance energy transfer （FRET）of TβRI biosensor were observed in live ceils. Results showed that the FRET ratio of TβRI biosensor was maximized at 6 minutes when the ligand of TGFβ1 was added. The maximum level was kept about 9 minutes ofterward, FRET ratios begin to decrease. Our study showed that the TGFβ1/ TβRI signal transduction pathway might have a characteristic of time delay in living ceils.