中文摘要：

目的：建立反相高效液相色谱法同时测定茵陈蒿汤（茵陈、栀子、大黄）中滨蒿内酯、儿茶素、绿原酸、东莨菪内酯、金丝桃苷、秦皮乙素、京尼平苷、槲皮素、京尼平、大黄酸、芦荟大黄素、大黄酚、大黄素、大黄素甲醚的含量。方法：采用Eclipse Plus C_（18）（250 mm×4.6 mm,5μm）色谱柱,以乙腈（A）-0.1%磷酸水溶液（B）为流动相,梯度洗脱,流速1.0mL·min（-1）,柱温30℃,检测波长210 nm（儿茶素、绿原酸、秦皮乙素、京尼平、东莨菪内酯、滨蒿内酯、槲皮素）、238 nm（京尼平苷、金丝桃苷）、254 nm（芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚）,进样量25μL。结果：14个成分可实现完全分离,在一定的线性范围内呈良好的线性关系（r〉0.999 8）、精密度（RSD≤2.0%）、稳定性（RSD≤1.9%）和重复性（RSD≤1.8%）良好,平均回收率（n=6）在94.4%-98.0%范围内,RSD在0.86%-2.8%范围内。3批茵陈蒿汤中滨蒿内酯、儿茶素、绿原酸、东莨菪内酯、金丝桃苷、秦皮乙素、京尼平苷、槲皮素、京尼平、大黄酸、芦荟大黄素、大黄酚、大黄素、大黄素甲醚14个成分的含量测定结果分别为0.136-0.168、0.080-0.104、0.403-0.470、0.008-0.009、0.061-0.068、0.020-0.027、0.269-0.306、0.036-0.048、0.005-0.006、0.122-0.164、0.037-0.044、0.030-0.037、0.011-0.014、0.007-0.010 mg·g（-1）。结论：该方法经方法学验证,可用于茵陈蒿汤中14个化学成分的含量测定。

英文摘要：

Objective：To establish a RP-HPLC method for simultaneous determination of scoparone,catechin,chlorogenic acid,scopoletin,hyperoside,esculetin,geniposide,quercetin,genipin,rhein,aloe-emodin,chrysophanol,emodin and physcion in Yin-Chen-Hao-Tang（Artemisia capillaris Thunb.,Gardenia jasminoidesEllis and Rheum PalmatumL.）.Methods：The separation was carried out on an Eclipse Plus C_（18）（250 mm×4.6 mm,5 μm）column eluted with the mobile phase of acetonitrile（A）-0.1% phosphoric acid aqueous solution（B）in a gradient elution manner at a flow rate of 1.0mL·min（-1）.The detection wavelength was set at 210 nm for scoparone,catechin,chlorogenic acid,scopoletin,esculetin,quercetin and genipin,at 238 nm for geniposide and hyperoside,and at 254 nm for rhein,aloe-emodin,chrysophanol,emodin and physcion.The column temperature was set at 30 ℃ and the injection volume was 25 μL.Results：Satisfactory separation was obtained for the 14 compounds.In the given concentration range,all the compounds showed good linear correlation（r0.999 8）.The RSD values of precision,stability and repeatability were lower than 2.0%,1.9% and 1.8%,respectively.The average recoveries of the 14 compounds were 94.4%-98.0% with the RSD values of 0.86%-2.8%（n=6）.The contents in 3 samples were in the ranges of 0.136-0.168 mg·g（-1） for scoparone,0.080-0.104 mg·g（-1） for catechin,0.403-0.470 mg·g（-1） for chlorogenic acid,0.008-0.009 mg·g（-1） for scopoletin,0.061-0.068 mg·g（-1） for hyperoside,0.020-0.027 mg·g（-1） for esculetin,0.269-0.306 mg·g（-1） for geniposide,0.036-0.048 mg·g（-1） for quercetin,0.005-0.006 mg·g（-1） for genipin,0.122-0.164 mg·g（-1） for rhein,0.037-0.044 mg·g（-1） for aloe-emodin,0.030-0.037 mg·g（-1） for chrysophanol,0.011-0.014 mg·g（-1） for emodin and 0.007-0.010 mg·g（-1） for physcion.Conclusion：This method was proved by methodology validation that it is suitable for the determination of those14 constituents in Yin-Chen-Hao-Tang.