中文摘要：

目的：研究体外扩增人类CD8＋记忆T细胞的新方法,为抗病毒与抗肿瘤的过继性免疫治疗提供新的手段。方法：将anti-CD3抗体、anti-CD28抗体、CD70、白细胞介素（IL）-2、IL-7和IL-15进行排列组合,设计出63种刺激方式,对体外分离得到的正常人外周血CD8＋T细胞进行体外扩增;培养14 d后进行细胞计数,并检测CD8＋T细胞的纯度以及CD8＋中枢记忆T细胞（TCM）和CD8＋效应记忆T细胞（TEM）所占的比例,进而计算出CD8＋T细胞、CD8＋TCM和CD8＋TEM的体外扩增倍数,从而确定理想的刺激方法。结果：体外扩增CD8＋T细胞、CD8＋TCM和CD8＋TEM的理想刺激方式均为anti-CD3抗体、IL-2和IL-7三者的组合;该刺激方式使3种细胞在培养14 d后分别扩增了13.19、13.28和15.27倍。结论：Anti-CD3抗体、IL-2和IL-7三者的组合,是刺激人类CD8＋记忆T细胞体外扩增的相对理想方法。

英文摘要：

AIM： To investigate new methods for the expansion of human CD8＋ memory T cells in vitro and provide novel means of anti-viral and anti-tumor adoptive immunotherapy.METHODS： Six kinds of stimuli,anti-CD3 antibody,anti-CD28 antibody,CD70,IL-2,IL-7 and IL-15,for the expansion of human CD8＋ memory T cells in vitro were selected and arranged for their combinations,resulting in 63 kinds of stimulating combinations.Normal human CD8＋ T cells were isolated and exposed to these stimuli.After 14 days of cell culture,the number and purity of CD8＋ T cells,and the percentages of CD8＋ central memory T cells（TCM） and CD8＋ effector memory T cells（TEM） were detected.The expansion folds of CD8＋ T cells,CD8＋ TCM and CD8＋ TEM were calculated and the relatively better stimulating combination was determined.RESULTS： The combination of anti-CD3 antibodies,IL-2 and IL-7 was a better method for the expansion of human CD8＋ T cells,CD8＋ TCM and CD8＋ TEM in vitro,and the expansion folds were 13.19,13.28 and 15.27,respectively.CONCLUSION： The combination of anti-CD3 antibodies,IL-2 and IL-7 is the relatively better method for the expansion of human CD8＋ memory T cells in vitro.