中文摘要：

目的：探讨小分子药物维生素c（VC）对CD4＋效应记忆性T细胞（TEM）体外扩增的影响从而改善过继免疫治疗的效果。方法：体外分离正常人的外周血CD4＋T淋巴细胞，将细胞分为2组（实验组和对照组）进行细胞培养。实验组加入VC后，通过细胞计数仪和流式细胞仪对2组细胞中TEM的扩增情况进行检测。结果：（1）在CD4＋T细胞体外扩增实验中，VC对CD4＋T细胞的总数无显著影响。（2）VC使TEM在CD4＋T细胞中的比例显著升高，100mg／L为扩增的最佳浓度。（3）在CD4＋T细胞扩增的第10天检测其中TEM的细胞数量，对照组TEM的数量为（1．22±0．15）×10^6，实验组TEM的数量为（3．56±0．35）×10^6，两者的差异有统计学意义（P〈0．01）。结论：VC可有效地促进CD4＋TEM的体外扩增，为过继免疫治疗提供简单、安全及有效的体外扩增方法。

英文摘要：

AIM： To investigate the effect of vitamin C （VC）, a small-molecule compound, on the expansion of CD4＋ effector memory T cells （TEM） in vitro and to improve the effect of adoptive immunotherapy. METHODS ： Normal human peripheral blood CD4＋ T-lymphocytes were isolated and randomly divided into 2 groups： control group and experiment group. VC was added into experimental group. Thereafter, the expansion of CD4＋ TEM in the 2 groups was detected by cell counter and flow cytometry. RESULTS： VC did not significantly affect the total number of CD4＋ T cells, while it raised the ratio of TEM in CD4＋ T cells at an optimal concentration of 100 mg/L. After 10 days of the expansion of CD4 ＋ T cells, the number of CD4＋ TEM in experiment group was （3.56 ±0.35 ）×10^6, significantly larger than that in control group [ （ 1.22 ± 0.15 ） ×10^6, P 〈 0.01 ]. CONCLUSION： VC can effectively promote the expansion of CD4＋ TEM in vitro, which provides a simple, safe and effective expansion method for adoptive immunotherapy. [