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中文摘要：
根据编码鼠伤寒沙门氏菌肠毒素stn基因的核苷酸序列设计1对引物和荧光探针，通过对荧光定量PCR反应体系和反应条件的摸索，建立了检测沙门氏菌的核酸荧光定量PCR方法。对该方法的特异性与敏感性研究结果显示，该方法检测沙门氏菌结果均为阳性，而非沙门氏菌均为阴性；对带有沙门氏菌肠毒素stn基因的阳性质粒的检测敏感性为4个／μL。用该方法对人工污染沙门氏菌的鲜猪肉和鲜鸡蛋进行检测，当检样中沙门氏菌初始含菌量分别为1CFU／g（鲜猪肉）和1CFU／g（鲜鸡蛋），经过12h的增菌后，检测结果均为阳性。该方法具有简便、快速、特异性强、敏感性高等特点。此研究为食品中沙门氏菌快速检测试剂盒的研制打下了良好的基础。
英文摘要：
A real-time PCR assay for detection of nucleotide of Salmonella typhimurium was established using a pair of primers and a probe designed according to the published nucleotide sequence of stn gene that encodes the entertoxin protein of Salmonella typhimurium. The assay had a detection limit of 4 copies/μL （100 copy/25 μL） for positive plasmid, and 1 CFU/g and 1 CFU/g for artificially contaminated fresh, meat and eggs, respectively. This method was rapid, highly sensitive and specific, which could be developed into a commercial kit for rapid detection of Salmonella typhimurium contaminations in food.
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